Chuanying Fang Hainan University Haikou
1 protocol

Kang Li Hainan University Haikou
1 protocol

Yuhui Wang
  • Hainan University Haikou
  • 1 Author merit
1 Protocol published
一种通过单轮PCR扩增构建用于植物转化的二元CRISPR载体的新方法
Authors:  Kang Li, Yuhui Wang and Chuanying Fang, date: 04/05/2021, view: 2657, Q&A: 0

CRISPR/Cas9 is an established and flexible tool for genome editing. However, most methods used to generate expression clones for the CRISPR/Cas9 are time-consuming. Hence, we have developed a one-step protocol to introduce sgRNA expression

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