Mafalda Escobar-Henriques University of Cologne Cologne
2 protocols

Tânia Simões University of Cologne Cologne
2 protocols

Ira Buntenbroich
  • Post-Doc, University of Cologne Cologne
Research focus
  • Mitochondrial dynamics, Ubiquitylation
  • 1 Author merit


Ph.D., University of Cologne, 2020

Lab information

Mafalda Escobar Lab

2 Protocols published
Analysis of Protein Stability by Synthesis Shutoff
Authors:  Ira Buntenbroich, Tânia Simões and Mafalda Escobar-Henriques, date: 11/20/2021, view: 813, Q&A: 0

In this protocol, we describe the analysis of protein stability over time, using synthesis shutoff. As an example, we express HA-tagged yeast mitofusin Fzo1 in Saccharomyces cerevisiae and inhibit translation via cycloheximide (CHX). Proteasomal

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