Rhoderick Brown University of Minnesota
1 protocol

Dinshaw Pate Memorial Sloan-Kettering Cancer Center
1 protocol

Charles Chalfant
1 protocol

Ngoc Vu
1 protocol

Lucy Malinina
Research focus
  • 1 Author merit
1 Protocol published
Purification of Cytosolic Phospholipase A2α C2-domain after Expression in Soluble Form in Escherichia coli

Previous expression/purification strategies for cytosolic phospholipase A2α C2-domain in Escherichia coli have relied on refolded protein recovered from inclusion bodies and sometimes containing C-terminal Cys139Ala and Cys141Ser substitutions

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