James Wakefield Living Systems Institute, University of Exeter, Stocker Road, EX4 4QD Exeter
1 protocol

Christian Soeller Living Systems Institute, University of Exeter, Stocker Road, EX4 4QD Exeter
2 protocols

Lucy Green Living Systems Institute, University of Exeter, Stocker Road, EX4 4QD Exeter
1 protocol

Ammarah Tariq
  • Living Systems Institute, University of Exeter, Stocker Road, EX4 4QD Exeter
  • 1 Author merit
1 Protocol published
Cleavable Affinity Purification (Cl-AP): A One-step Procedure to Affinity Purify Protein Complexes
Authors:  Ammarah Tariq, Lucy Green, Christian Soeller and James G. Wakefield, date: 11/20/2020, view: 112, Q&A: 0

Cleavable Affinity Purification (Cl-AP) uses a tripartite system of Protein-A-Streptavidin beads and nanobodies, coupled with a biotinylated, thiol-cleavable linker, providing one-step affinity purification from lysates of tissues expressing tagged

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