Kedar Narayan Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, NIH Frederick
1 protocol

Irene Chang Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD
1 protocol

Mohammad Rahman NIH Bethesda
2 protocols

Orna Cohen-Fix
  • National Institute for Diabetes and Digestive and Kidney Diseases, NIH Bethesda
Research focus
  • Cell biology
  • 1 Author merit
1 Protocol published
A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy
Authors:  Mohammad M. Rahman, Irene Y. Chang, Orna Cohen-Fix and Kedar Narayan, date: 04/05/2021, view: 2814, Q&A: 0

The free-living nematode Caenorhabditis elegans is a popular model system for studying developmental biology. Here we describe a detailed protocol to high-pressure freeze the C. elegans embryo (either ex vivo after dissection, or within the intact

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