Jianjun Sun University of Texas at El Paso El Paso
2 protocols

Salvador Vazquez-Reyes University of Texas at El Paso El Paso
1 protocol

Javier Aguilera
  • Research associate, University of Texas at El Paso El Paso
  • 2 Author merit

Lab information

Sun Lab

2 Protocols published
A Fluorescence Dequenching-based Liposome Leakage Assay to Measure Membrane Permeabilization by Pore-forming Proteins
Authors:  Javier Aguilera, Salvador Vazquez-Reyes and Jianjun Sun, date: 05/20/2021, view: 2641, Q&A: 0

Pore-forming toxins (PFTs) have been discovered in a wide range of organisms. Their functions are essential to the survival or virulence of many species. PFTs often interact with lipid membranes. Large unilamellar vesicles (LUV), also known as

...
Measuring Cytosolic Translocation of Mycobacterium marinum in RAW264.7 Macrophages with a CCF4-AM FRET Assay
Authors:  Javier Aguilera and Jianjun Sun, date: 04/20/2021, view: 1316, Q&A: 0
The CCF4-AM Förster resonance energy transfer (FRET) assay is a sensitive approach to measure bacterial cytosolic translocation in live cells. The FRET pair hydroxycoumarin (donor) and fluorescein (acceptor) are linked by a CCF4-AM β-lactam ring, ...
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