Jianjun Sun University of Texas at El Paso El Paso
1 protocol

Salvador Vazquez-Reyes University of Texas at El Paso El Paso
0 protocol

Javier Aguilera
  • Research associate, University of Texas at El Paso El Paso
  • 1 Author merit

Lab information

Sun Lab

1 Protocol published
Measuring Cytosolic Translocation of Mycobacterium marinum in RAW264.7 Macrophages with a CCF4-AM FRET Assay
Authors:  Javier Aguilera and Jianjun Sun, date: 04/20/2021, view: 484, Q&A: 0
The CCF4-AM Förster resonance energy transfer (FRET) assay is a sensitive approach to measure bacterial cytosolic translocation in live cells. The FRET pair hydroxycoumarin (donor) and fluorescein (acceptor) are linked by a CCF4-AM β-lactam ring, ...
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