Khaled Selim Tübingen University Tübingen
1 protocol

Karl Forchhammer
2 protocols

Tim Orthwein
1 protocol

Luciano F. Huergo
  • Faculty,
  • 1 Author merit
1 Protocol published
Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (KD) and the Effective Concentration (EC50) of an Interplaying Effector Molecule Using Bio-layer Interferometry
Authors:  Tim Orthwein, Luciano F. Huergo, Karl Forchhammer and Khaled A. Selim, date: 09/05/2021, view: 1428, Q&A: 0

Biolayer interferometry (BLI) is an emerging analytical tool that allows the study of protein complexes in real time to determine protein complex kinetic parameters. This article describes a protocol to determine the KD of a protein complex using a

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