Yuhui Wang Hainan University Haikou
1 protocol

Kang Li Hainan University Haikou
1 protocol

Chuanying Fang
  • Research scientist, Hainan University Haikou
Research focus
  • Plant science
  • 1 Author merit
1 Protocol published
A Novel Method to Construct Binary CRISPR Vectors for Plant Transformation by Single Round of PCR Amplification
Authors:  Kang Li, Yuhui Wang and Chuanying Fang, date: 04/05/2021, view: 3159, Q&A: 0

CRISPR/Cas9 is an established and flexible tool for genome editing. However, most methods used to generate expression clones for the CRISPR/Cas9 are time-consuming. Hence, we have developed a one-step protocol to introduce sgRNA expression

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