Zhenyi Hong —BIO-PROTOCOL

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Lino Tessarollo Neural Development Section, Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick
1 protocol

Gianluca Fulgenzi 1 protocol

Francesco Tomassoni-Ardori Neural Development Section, Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick
1 protocol

Zhenyi Hong

Neural Development Section, Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick

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1 Protocol published

Generation of Functional Mouse Hippocampal Neurons

Authors: Francesco Tomassoni-Ardori, Zhenyi Hong, Gianluca Fulgenzi and Lino Tessarollo, date: 08/05/2020, view: 2775, Q&A: 1

Primary culture of mouse hippocampal neurons is a very useful in vitro model for studying neuronal development, axonal and dendritic morphology, synaptic functions, and many other neuronal features. Here we describe a step-by-step process ...

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Victoria Hewitt

Columbia University

Thanks for sharing this protocol. Have you ever done any staining to confirm you do not end up with any cortical neurons in your prep? If so could you share which markers you used?

6/14/2021 8:21:54 AM Reply

Victoria Hewitt

Columbia University

Thanks for getting back to me - I'm trying to pair this with an electroporation before plating at E15.5 so the dissection is a little more fiddly but they grow a lot more robustly than the cortical neurons. It would just be nice to have a way to be sure.
Thanks
Victoria

2021-06-16 09:32:48

Francesco Tomassoni-Ardori

Neural Development Section, Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick

Hi Victoria! The straight answer to you question is no, we have never tried any specific cortical marker on those cultures. The hippocampus has a very well defined structure which allows a clean dissection (as you can see in Fig.6). Being the "starting material" so pure, you would expect a minimal or negligible cortical contamination, if any. All the best!

2021-06-15 06:57:25