Ariel Diaz —BIO-PROTOCOL

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Manuel Yepes Yerkes National Primate Research Center Atlanta
1 protocol

Enrique Torre Yerkes National Primate Research Center Emory University
1 protocol

Ariel Diaz

Yerkes National Primate Research Center Atlanta

Research focus

Neuroscience

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Preparation of Synaptoneurosomes to Study the Synapse in the Murine Cerebral Cortex

Authors: Ariel Diaz, Enrique Torre and Manuel Yepes, date: 01/20/2021, view: 944, Q&A: 1

The synapse is a complex structure where the transmission of information takes place. Synaptic dysfunction is one of the earliest pathophysiological events in several diseases, such as traumatic brain injury, cerebral ischemia, and neurodegenerative

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Preeti Kute

University of Bergen

Hello,
Thanks for the protocol. You mention that these SN can be stored at -80 for a year. What is the exact application of these SNs? Have you also done an EM to check the integrity of these synaptoneurosomes? Also, people use synaptoneurosomes for synaptic stimulation, have you tried the same after this protocol?
Best,
Preeti

2/12/2021 3:05:28 AM Reply

Ariel Diaz

Yerkes National Primate Research Center Atlanta

Hi Preeti,

Thank you for reading our protocol. These SNs can be used in all of the applications you mentioned; however, if you store them at -80, you will compromise the SNs integrity. If you want to perform functional and structural studies, you should use fresh SNs. Additionally, the EGTA in the buffers can be decreased or removed to reduce the interference with calcium signaling (the protease activity will increase too).

Best,

Ariel

2021-02-19 06:16:35