Orsolya Kekesi Biomedicine Discovery Institute & Department of Physiology, Monash University
1 protocol

Yossi Buskila
  • Senior Lecturer, School of Medicine, Western Sydney University, 2012-2020
Research focus
  • -
  • 1 Author merit

Education

PhD, Ben-Gurion University, Israel, 2010

Publications

• Kékesi, O., Liang, H., Münch, G., Morley, J. W., Gyengesi, E. and Buskila, Y. (2019). The differential impact of acute microglia activation on the excitability of cholinergic neurons in the mouse medial septum. Brain Structure and Function 224(7): 2297-2309.
• Bellot-Saez, A., Kékesi, O., Ben-Abu, Y., Morley, J. W. and Buskila, Y. (2019). Neuromodulation of astrocytic K+ clearance. bioRxiv: 841643.
• Buskila, Y., Bellot-Saez, A. and Morley, J. W. (2019). Generating Brain Waves, the Power of Astrocytes. Front Neurosci 13: 1125.
• Kekesi, O., Liang, H., Munch, G., Morley, J. W., Gyengesi, E. and Buskila, Y. (2019). The differential impact of acute microglia activation on the excitability of cholinergic neurons in the mouse medial septum. Brain Struct Funct 224(7): 2297-2309.
• Buskila, Y., Kékesi, O., Bellot-Saez, A., Seah, W., Berg, T., Trpceski, M., Yerbury, J. J. and Ooi, L. (2019). Dynamic interplay between H-current and M-current controls motoneuron hyperexcitability in amyotrophic lateral sclerosis. Cell Death & Disease 10(4): 310.
• Bellot-Saez, A., Cohen, G., van Schaik, A., Ooi, L., J, W. M. and Buskila, Y. (2018). Astrocytic modulation of cortical oscillations. Sci Rep 8(1): 11565.
• Bellot-Saez, A., Kékesi, O., Morley, J. W. and Buskila, Y. (2017). Astrocytic modulation of neuronal excitability through K+ spatial buffering. Neuroscience and biobehavioral reviews 77: 87-97.
• Do-Ha, D., Buskila, Y. and Ooi, L. (2018). Impairments in Motor Neurons, Interneurons and Astrocytes Contribute to Hyperexcitability in ALS: Underlying Mechanisms and Paths to Therapy. Mol Neurobiol 55(2): 1410-1418.
• Cameron, M. A., Al Abed, A., Buskila, Y., Dokos, S., Lovell, N. H. and Morley, J. W. (2017). Differential effect of brief electrical stimulation on voltage-gated potassium channels. J Neurophysiol 117(5): 2014-2024.
• Cameron, M. A., Kekesi, O., Morley, J. W., Bellot-Saez, A., Kueh, S., Breen, P., van Schaik, A., Tapson, J. and Buskila, Y. (2017). Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging. J Vis Exp(120).
• Cameron, M., Kekesi, O., Morley, J. W., Tapson, J., Breen, P. P., van Schaik, A. and Buskila, Y. (2016). Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. PLoS One 11(5): e0155468.
• Zaman, M. S., Johnson, A. J., Bobek, G., Kueh, S., Kersaitis, C., Bailey, T. D., Buskila, Y. and Wu, M. J. (2016). Protein kinase CK2 regulates metal toxicity in neuronal cells. Metallomics 8(1): 82-90.
• Buskila, Y., Breen, P. P., Tapson, J., van Schaik, A., Barton, M. and Morley, J. W. (2014). Extending the viability of acute brain slices. Sci Rep 4: 5309.
• Breen, P. P. and Buskila, Y. (2014) Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology. Engineering in Medicine and Biology Society (EMBC), 36th Annual International Conference of the IEEE ,pp.4864,4867
• Buskila, Y., Morley, J. W., Tapson, J. and van Schaik, A. (2013). The adaptation of spike backpropagation delays in cortical neurons. Front Cell Neurosci 7: 192.
• Buskila, Y., Crowe, S. E. and Ellis-Davies, G. C. (2013). Synaptic deficits in layer 5 neurons precede overt structural decay in 5xFAD mice. Neuroscience 254: 152-159.
• Tapson, J. C., Cohen, G. K., Afshar, S., Stiefel, K. M., Buskila, Y., Wang, R. M., Hamilton, T. J. and van Schaik, A. (2013). Synthesis of neural networks for spatio-temporal spike pattern recognition and processing. Front Neurosci 7: 153.
• Shlosberg, D., Buskila, Y., Abu-Ghanem, Y. and Amitai, Y. (2012). Spatiotemporal alterations of cortical network activity by selective loss of NOS-expressing interneurons. Front Neural Circuits 6: 3.
• Kantevari, S., Buskila, Y. and Ellis-Davies, G. C. (2012). Synthesis and characterization of cell-permeant 6-nitrodibenzofuranyl-caged IP3. Photochem Photobiol Sci 11(3): 508-513.
• Buskila, Y. and Amitai, Y. (2010). Astrocytic iNOS-dependent enhancement of synaptic release in mouse neocortex. J Neurophysiol 103(3): 1322-1328.
• Kantevari, S., Buskila, Y. and Ellis-Davies, G. C. (2012). Synthesis and characterization of cell-permeant 6-nitrodibenzofuranyl-caged IP3. Photochem Photobiol Sci 11(3): 508-513.
• Buskila, Y. and Amitai, Y. (2010). Astrocytic iNOS-dependent enhancement of synaptic release in mouse neocortex. J Neurophysiol 103(3): 1322-1328.
• Abu-Ghanem, Y., Cohen, H., Buskila, Y., Grauer, E. and Amitai, Y. (2008). Enhanced stress reactivity in nitric oxide synthase type 2 mutant mice: findings in support of astrocytic nitrosative modulation of behavior. Neuroscience 156(2): 257-265.
• Buskila, Y., Abu-Ghanem, Y., Levi, Y., Moran, A., Grauer, E. and Amitai, Y. (2007). Enhanced astrocytic nitric oxide production and neuronal modifications in the neocortex of a NOS2 mutant mouse. PLoS One 2(9): e843.
• Buskila, Y., Farkash, S., Hershfinkel, M. and Amitai, Y. (2005). Rapid and reactive nitric oxide production by astrocytes in mouse neocortical slices. Glia 52(3): 169-176.
• Shlosberg, D., Patrick, S. L., Buskila, Y. and Amitai, Y. (2003). Inhibitory effect of mouse neocortex layer I on the underlying cellular network. Eur J Neurosci 18(10): 2751-2759.
1 Protocol published
Method for Prolonged Incubation of Brain Slices
Authors:  Orsolya Kekesi and Yossi Buskila, date: 07/20/2020, view: 220, Q&A: 0
Slices of neuronal tissue maintain a high degree of topographical and functional properties of neurons and glia and therefore are extensively used for measurements of neuronal activity at the molecular, cellular and network levels. However, the ...
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