James Shorter Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, USA
2 protocols

Ryan Richard Cupo
Research focus
  • Microbiology
  • 1 Author merit
1 Protocol published
Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli
Authors:  Ryan R. Cupo and James Shorter, date: 12/05/2020, view: 1404, Q&A: 0

Skd3 (encoded by human CLPB) is a mitochondrial AAA+ protein comprised of an N-terminal ankyrin-repeat domain and a C-terminal HCLR-clade nucleotide-binding domain. The function of Skd3 has long remained unknown due to challenges in purifying the

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