Dear YX L,

Thank you for your inquiry.

We use single molecule FISH probes from Biosearch and we plan them using the Biosearch probe design engine (https://www.biosearchtech.com/stellaris-designer).

In terms of target specificity concerns - typically, we plan sets of 24-48 probes per gene, at the highest masking level (level 5 - highest specificity). The Biosearch designer "checks" the specificity and cross reactivity of probes, and it should suggest probes that do not cross react (again, depending on the masking level you choose). As such, the specificity and accuracy is extremely high, as in single molecule FISH.

Otherwise, our protocol in terms of hybridization of the probes is based on the single molecule FISH protocol, which has been shown extensively in the literature to work (probes binding their targets) for a variety of genes.

Hope this addresses your concern? Please write back if it doesn't.

Thank you again for your inquiry.

Anca 2021-06-08 02:03:00