Yu Jin Department of Plant and Environmental Sciences University of Copenhagen
1 protocol

Sebastian Marquardt
  • Faculty, University of Copenhagen Frederiksberg
Research focus
  • Plant science
  • Epigenetics, Non-coding DNA, Gene expression, long non-coding RNA, budding yeast, plant biology
  • 1 Author merit
1 Protocol published
Dual sgRNA-based Targeted Deletion of Large Genomic Regions and Isolation of Heritable Cas9-free Mutants in Arabidopsis
Authors:  Yu Jin and Sebastian Marquardt, date: 10/20/2020, view: 563, Q&A: 0
CRISPR/Cas9 system directed by a gene-specific single guide RNA (sgRNA) is an effective tool for genome editing such as deletions of few bases in coding genes. However, targeted deletion of larger regions generate loss-of-function alleles ...
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