Ryan Cupo
1 protocol

Lin Guo Biochemistry and Molecular Biology, Thomas Jefferson University, 2019-
1 protocol

Emma Robinson Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 2019-2020
1 protocol

James Shorter
  • Professor, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, USA
Research focus
  • Biochemistry
  • 2 Author merit


Ph.D., University of London, U.K.


2 Protocols published
Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli
Authors:  Ryan R. Cupo and James Shorter, date: 12/05/2020, view: 2141, Q&A: 0

Skd3 (encoded by human CLPB) is a mitochondrial AAA+ protein comprised of an N-terminal ankyrin-repeat domain and a C-terminal HCLR-clade nucleotide-binding domain. The function of Skd3 has long remained unknown due to challenges in purifying the

Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUS
Authors:  Emma Robinson, James Shorter and Lin Guo, date: 08/20/2020, view: 2515, Q&A: 0
The study of RNA-binding proteins (RBP) offers insight into the mechanisms of pathologic protein aggregation in neurodegenerative diseases. We developed a protocol for purifying an RBP FUS and a nuclear import receptor (NIR) Kapβ2 and testing the ...
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