Alessandro Prigione —BIO-PROTOCOL

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Pawel Lisowski Heinrich Heine University Düsseldorf
1 protocol

Annika Zink Heinrich Heine University Düsseldorf
1 protocol

Alessandro Prigione

Faculty, Heinrich Heine University Düsseldorf

Research focus

Neuroscience
iPSC-based modeling of mitochondrial diseases

1 Author merit
Reviewer merit
Editor merit

Lab information

Prigione Lab: "Stem Cell Metabolism"
http://www.uniklinik-duesseldorf.de/prigione-labor

1 Protocol published

Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders

Authors: Annika Zink, Pawel Lisowski and Alessandro Prigione, date: 03/05/2021, view: 2274, Q&A: 1

The high attrition rate in drug development processes calls for additional human-based model systems. However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced

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Pashma Nawaz

University of Central Punjab

what is M3 (sm+)? and what stands for sm+ ?

5/3/2021 9:03:45 AM Reply

Annika Zink

Heinrich Heine University Düsseldorf

Dear Pashma,

hadia mujahid is right. M3, also called sm+, is the final culturing media for the NPCs. Once the generation of the NPCs is completed, cells are kept in sm+ throughout the culture. You can find the media composition in Tab. 1 (basic media) and Tab. 2.

2021-05-10 01:15:28

hadia mujahid

this is a media used for culturing the neural cells. it could be used for freezing, moreover you can look for the functions through the composition provided in this article.

2021-05-08 22:11:24