Holden Maecker Human Immune Monitoring Center (HIMC), Institute for Immunity, Transplantation and Infection, Department of Microbiology & Immunology, Stanford University, USA
9 protocols

Sangeeta Kowli
  • 1 Author merit
1 Protocol published
Intracellular Staining on Fixed Whole Blood for Mass Cytometry (CyTOF)
Authors:  Sangeeta Kowli and Holden Maecker, date: 04/23/2020, view: 247, Q&A: 0
In this report, we present the implementation of mass cytometry for intracellular staining using fixed whole blood. In our assay described here, 250 µl of whole blood, is stimulated in vitro with PMA/ionomycin (or left unstimulated), in the ...
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