Tatsuo Fukagawa —BIO-PROTOCOL

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Kohei Nishimura Nagoya Nagoya
1 protocol

Mariko Ariyoshi Graduate School of frontier biosciences, Osaka University
1 protocol

Masatoshi Hara Graduate School of Frontier Biosciences Osaka Univ
1 protocol

Reito Watanabe Graduate School of Frontier Biosciences Osaka Univ
1 protocol

Tatsuo Fukagawa

Prof.,

Research focus

Kinetochore: Centromere; Mitosis; chromosome segregation; Epigenetics

2 Author merit
Reviewer merit
Editor merit

Education

Ph. D, National Institute Genetics, 1995

Lab information

Fukagawa Lab
http://www.fbs.osaka-u.ac.jp/labs/fukagawa/index.html

2 Protocols published

A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines

Authors: Kohei Nishimura and Tatsuo Fukagawa, date: 07/20/2021, view: 428, Q&A: 0

Inducing loss of function of a target protein using methods such as gene knockout is a powerful and useful strategy for analyzing protein function in cells. In recent years, the CRISPR/Cas-9-based gene knockout technology has been widely used across

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CENP-C Phosphorylation by CDK1 in vitro

Authors: Reito Watanabe, Masatoshi Hara, Mariko Ariyoshi and Tatsuo Fukagawa, date: 01/05/2021, view: 1989, Q&A: 0

Accurate chromosome segregation during mitosis requires the kinetochore, a large protein complex, which makes a linkage between chromosomes and spindle microtubes. An essential kinetochore component, CENP-C, is phosphorylated by Cyclin-B-Cyclin

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