Kohei Nishimura Nagoya Nagoya
1 protocol

Mariko Ariyoshi Graduate School of frontier biosciences, Osaka University
1 protocol

Masatoshi Hara Graduate School of Frontier Biosciences Osaka Univ
1 protocol

Reito Watanabe Graduate School of Frontier Biosciences Osaka Univ
1 protocol

Tatsuo Fukagawa
  • Prof.,
Research focus
  • Kinetochore: Centromere; Mitosis; chromosome segregation; Epigenetics
  • 2 Author merit

Education

Ph. D, National Institute Genetics, 1995

Lab information

Fukagawa Lab
http://www.fbs.osaka-u.ac.jp/labs/fukagawa/index.html

2 Protocols published
A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines
Authors:  Kohei Nishimura and Tatsuo Fukagawa, date: 07/20/2021, view: 428, Q&A: 0

Inducing loss of function of a target protein using methods such as gene knockout is a powerful and useful strategy for analyzing protein function in cells. In recent years, the CRISPR/Cas-9-based gene knockout technology has been widely used across

...
CENP-C Phosphorylation by CDK1 in vitro
Authors:  Reito Watanabe, Masatoshi Hara, Mariko Ariyoshi and Tatsuo Fukagawa, date: 01/05/2021, view: 1989, Q&A: 0

Accurate chromosome segregation during mitosis requires the kinetochore, a large protein complex, which makes a linkage between chromosomes and spindle microtubes. An essential kinetochore component, CENP-C, is phosphorylated by Cyclin-B-Cyclin

...
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