Dario Krapf Laboratory of Cell Signal Transduction Networks, Instituto de Biologia Molecular y Celular de Rosario, Rosario
1 protocol

Pablo Visconti Department of Veterinary and Animal Sciences, Umass, Amherst
1 protocol

Carolina Baro Graf Laboratoty of Cell Signal Transduction Network, Institute of Molecular and Cellular Biology of Rosario (IBR), 2016-
1 protocol

Cintia Stival
  • Research Associate, Cell Signal Transduction Networks, Institute of Molecular and Cellular Biology of Rosario
Research focus
  • -
  • 1 Author merit


PhD in Biological Sciences, Universidad Nacional de Rosario


• Baro Graf, C., Ritagliati, C., Torres-Monserrat, V., Stival, C., Carizza, C., Buffone, M. G. and Krapf, D. (2019). Membrane Potential Assessment by Fluorimetry as a Predictor Tool of Human Sperm Fertilizing Capacity. Front Cell Dev Biol 7: 383.
• Baro Graf, C., Ritagliati, C., Stival, C., Balestrini, P. A., Buffone, M. G. and Krapf, D. (2019). Determination of a Robust Assay for Human Sperm Membrane Potential Analysis. Front Cell Dev Biol 7: 101.
• Ritagliati, C., Luque, G. M., Stival, C., Baro Graf, C., Buffone, M. G. and Krapf, D. (2018). Lysine acetylation modulates mouse sperm capacitation. Sci Rep 8(1): 13334.
• Stival, C., Ritagliati, C., Xu, X., Gervasi, M. G., Luque, G. M., Baro Graf, C., De la Vega-Beltran, J. L., Torres, N., Darszon, A., Krapf, D., Buffone, M. G., Visconti, P. E. and Krapf, D. (2018). Disruption of protein kinase A localization induces acrosomal exocytosis in capacitated mouse sperm. J Biol Chem 293(24): 9435-9447.
• Ritagliati, C., Baro Graf, C., Stival, C. and Krapf, D. (2018). Regulation mechanisms and implications of sperm membrane hyperpolarization. Mech Dev 154: 33-43.
• González, L. J., Stival, C., Puzzolo, J. L., Moreno, D. M. and Vila, A. J. (2018). Shaping Substrate Selectivity in a Broad-Spectrum Metallo-beta-Lactamase. Antimicrob Agents Chemother 62(4).
• Stival, C., Puga Molina Ldel, C., Paudel, B., Buffone, M. G., Visconti, P. E. and Krapf, D. (2016). Sperm Capacitation and Acrosome Reaction in Mammalian Sperm. Adv Anat Embryol Cell Biol 220: 93-106.
• La Spina, F. A., Stival, C., Krapf, D. and Buffone, M. G. (2017). Molecular and Cellular Aspects of Mammalian Sperm Acrosomal Exocytosis. In H. Schatten & GM. Constantinescu (Eds.), Animal Models and Human Reproduction (pp. 409–426). Hoboken, New Jersey: John Wiley & Sons Inc.
• Stival, C., La Spina, F. A., Baro Graf, C., Arcelay, E., Arranz, S. E., Ferreira, J. J., Le Grand, S., Dzikunu, V. A., Santi, C. M., Visconti, P. E., Buffone, M. G. and Krapf, D. (2015). Src Kinase Is the Connecting Player between Protein Kinase A (PKA) Activation and Hyperpolarization through SLO3 Potassium Channel Regulation in Mouse Sperm. J Biol Chem 290(30): 18855-18864.
1 Protocol published
Quantification of Protein Kinase A (PKA) Activity by An in vitro Radioactive Assay Using the Mouse Sperm Derived Enzyme
Authors:  Cintia Stival, Carolina Baro Graf, Pablo E. Visconti and Dario Krapf, date: 06/20/2020, view: 570, Q&A: 0
In order to acquire fertilizing potential, mammalian sperm must undergo a process known as capacitation, which relies on the early activation of Protein Kinase A (PKA). Frequently, PKA activity is assessed in whole-cell ...
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