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Nobumoto Watanabe
Bio-Active Compounds Discovery Research Unit, RIKEN CSRS, Saitama, 351-0198
2 protocols
Yasumitsu Kondoh
2 protocols
Makoto Muroi
2 protocols
Kaori Honda
2 protocols
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Hiroyuki Osada
Contributions
2
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2 Protocols published
Identification of Target Protein for Bio-active Small Molecule Using Photo-cross Linked Beads and MALDI-TOF Mass Spectrometry
Authors:
Kruthi Suvarna
,
Kaori Honda
,
Makoto Muroi
,
Yasumitsu Kondoh
,
Nobumoto Watanabe
and
Hiroyuki Osada
,
date:
02/05/2020,
view:
2070,
Q&A:
0
Development of methods for protein identification is one of the important aspects of proteomics. Here, we report a protocol for the preparation of compound conjugated beads by photo-crosslinking, affinity purification, gel electrophoresis, and ...
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Measurement of ATPase Activity of Valosin-containing Protein/p97
Authors:
Kruthi Suvarna
,
Kaori Honda
,
Makoto Muroi
,
Yasumitsu Kondoh
,
Hiroyuki Osada
and
Nobumoto Watanabe
,
date:
02/05/2020,
view:
2055,
Q&A:
0
Valosin-containing protein (VCP; also known as p97) is a type II ATPase regulating several cellular processes. Using proteomic techniques, we identified a chemical compound that binds to the D1 ATPase domain of VCP. The protocol described here was ...
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