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Nobumoto Watanabe Bio-Active Compounds Discovery Research Unit, RIKEN CSRS, Saitama, 351-0198
 2 protocols

Yasumitsu Kondoh 2 protocols

Makoto Muroi 2 protocols

Kaori Honda 2 protocols

Hiroyuki Osada
  • 2 Author merit
2 Protocols published
Identification of Target Protein for Bio-active Small Molecule Using Photo-cross Linked Beads and MALDI-TOF Mass Spectrometry
Authors:  Kruthi Suvarna, Kaori Honda, Makoto Muroi, Yasumitsu Kondoh, Nobumoto Watanabe and Hiroyuki Osada, date: 02/05/2020, view: 1118, Q&A: 0
Development of methods for protein identification is one of the important aspects of proteomics. Here, we report a protocol for the preparation of compound conjugated beads by photo-crosslinking, affinity purification, gel electrophoresis, and ...
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Measurement of ATPase Activity of Valosin-containing Protein/p97
Authors:  Kruthi Suvarna, Kaori Honda, Makoto Muroi, Yasumitsu Kondoh, Hiroyuki Osada and Nobumoto Watanabe, date: 02/05/2020, view: 1089, Q&A: 0
Valosin-containing protein (VCP; also known as p97) is a type II ATPase regulating several cellular processes. Using proteomic techniques, we identified a chemical compound that binds to the D1 ATPase domain of VCP. The protocol described here was ...
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