Peter Hitchcock University of Michigan Ann Arbor
1 protocol

Mikiko Nagashima
  • Research Investigator, Ophthalmology and Visual Sciences University of Michigan
Research focus
  • Developmental biology
  • 1 Author merit


PhD, Kanazawa University, 2009


Nagashima, M., Hitchcock. F.P. Whole-mount immunohistochemistry of adult zebrafish retina. (accepted)

Nunley, N., Nagashima, M., Martin, K., Gonzalez, A.L., Suzuki, S.C., Norton, D., Wong, R.O., Raymond, P.A., Lubensky, D.K. Defect patterns on the curved surface of fish retinae suggest mechanism of cone mosaic formation. doi: (accepted)

Lahne M, Nagashima M, Hyde RD, Hitchcock FP. 2020. Reprogramming Muller Glia to Regenerate Retinal Neurons. Annu. Rev. Vis. Sci. Vol. 6:-(Volume publication date September 2020) PMID: 32343929.

Saito, Y., Okyuyoshi, H., Nakamura, S., Otsu, Wataru., Yamaguchi, A., Hitchcock, P.F., Nagashima, M., Shimazawa, M., Hara, H. 2020. Tauroursodeoxycholic acid promotes neuronal survival and proliferation of tissue resident stem and progenitor cells in retina of adult zebrafish. BPB Reports 3:92-96.

Ang, N.B., Saera-Vila, A., Walsh, C., Hitchcock, P.F., Kahana, A., Thummel, R., Nagashima, M. 2020. Midkine-a functions as a universal regulator of proliferation during epimorphic regeneration in adult zebrafish. PLOS ONE. 15(6): e0232308. PMID: 32530962.

Nagashima, M., Travis S.D., Hesse, D., Sifuentes, C.J., Raymond, P.A., Hitchcock. P.F. Midkine-a Is Required for Cell Cycle Progression of Müller Glia during Neuronal Regeneration. J. Neurosci. 40: 1232-1247. PMID: 31882403.

Silva, N.J., Nagashima, M., Li, J., Kakuk-Atkins, L., Ashrafzadeh, M., Hyde, D.R., Hitchcock. F.P. Inflammation and Matrix Metalloproteinase 9 (Mmp-9) Regulate Photoreceptor Regeneration in Adult Zebrafish. Glia. 68: 1445-1495. PMID: 32034934.

Fu, J*., Nagashima, M*., Guo, C., Raymond, P.A., Wei, X. 2018. Novel animal model of Crumbs-dependent progressive retinal degeneration that targets specific cone subtypes. (*equal contribution) IOVS 59: 505-518. PMID: 29368007.

Nagashima, M., Hadidjojo, J., Barthel, L.K., Lubensky, D.K., Raymond, P.A. 2017. Müller glial scaffolding supports a multiplex lattice mosaic of photoreceptors in zebrafish retina. Neural Dev. 15:20. PMID: 29141686.

Raymond, P.A., Colvin, S.M., Jabeen, Z., Nagashima, M., Barthel, L.K., Hadidjojo, J., Popova, L., Pejaver, V.R., Lubensky, D.K. 2014. Patterning the cone mosaic array in zebrafish retina requires specification of ultraviolet-sensitive cones. PLOS ONE 21: e85325. PMID: 24465536

Ogai K, Kuwana A, Hisano S, Nagashima M, Koriyama Y, Sugitani K, Mawatari K, Nakashima H, Kato S. 2014. Upregulation of leukemia inhibitory factor (LIF) during the early stage of optic nerve regeneration in zebrafish. PLOS ONE. 27: e106010. PMID: 25162623

Nagashima M, Barthel LK, Raymond PA. 2013. A self-renewing division of zebrafish Müller glial cells generates neuronal progenitors that require N-cadherin to regenerate retinal neurons. Development 140: 4510-4521. PMID: 24154521.

Nagashima M, Fujikawa C, Mawtari K, Mori Y, Kato S. 2011. HSP70, the earliest-induced gene in the zebrafish retina during optic nerve regeneration: Its role in cell survival. Neurochem Int. 58: 888-895. PMID: 21338645.

Koriyama Y, Yasuda R, Homma K, Mawatari K, Nagashima M, Sugitani K, Matsukawa T, Kato S. 2009. Nitric oxide-cGMP signaling regulates axonal elongation during optic nerve regeneration in the goldfish in vitro and in vivo. J. Neurochem. 110: 890-901. PMID: 19457064.

Nagashima M, Mawatari K, Tanaka M, Higashi T, Saito H, Muramoto K, MatsukawaT, Koriyama Y, Sugitani K, Kato S. 2009. Purpurin is a key molecule for cell differentiation during the early development of zebrafish retina. Brain Res. 1302:54-63. PMID: 19748496.

Nagashima M, Sakurai H, Mawatari K, Koriyama Y, Matsukawa T, Kato S. 2009. Involvement of retinoic acid signaling in goldfish optic nerve regeneration. Neurochem. Int. 54: 229-236. PMID: 19114071.

Kaneda M, Nagashima M, Nunome T, Muramatsu T, Yamada Y, Kubo M, Muramoto K, Matsukawa T, Koriyama Y, Sugitani K, Vachkov I, Mawatari K, Kato S. 2008. Changes of phospho-growth associated protein 43 (phospho-GAP43) in zebrafish retina after optic nerve injury: a long term observation. Neurosci. Res. 61: 281-288. PMID: 1848550.

Tanaka M, Murayama D, Nagashima M, Higashi T, Mawatari K, Matsukawa T, Kato S. 2007. Purpurin expression in the zebrafish retina during early development and after optic nerve lesion in adults. Brain Res. 1153:34-42. PMID: 17466280.
1 Protocol published
Whole-mount Immunohistochemistry of Adult Zebrafish Retina for Advanced Imaging
Authors:  Mikiko Nagashima and Peter F. Hitchcock, date: 12/20/2020, view: 1947, Q&A: 0

Immunohistochemistry is a widely used technique to examine the expression and subcellular localization of proteins. This technique relies on the specificity of antibodies and requires adequate penetration of antibodies into tissues. The latter is

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