Chandrahasya Khobragade School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded
1 protocol

Bhagwan Rekadwad Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune
1 protocol

Juan M. Gonzalez
  • Research scientist, Institute of Natural Resources and Agrobiology, Spanish Council for Research
Research focus
  • -
  • 1 Author merit


PhD, Basque Country University, Spain


Since 2012
• Moxley, E., E. Puerta-Fernández, E.J. Gómez, J.M. González. (2019). Influence of the abiotic factors temperature and water content on bacterial 2-chlorophenol biodegradation in soils. Frontiers in Environmental Science 7: 41. doi:10.3389/fenvs.2019.00041.
• Diánez, F, F. Marín, M. Santos, F.J. Gea, M.J. Navarro, M. Piñeiro, J.M. González. (2018). Genetic analysis and in vitro enzymatic determination of bacterial community in compost teas from different sources. Compost Science & Utilization. doi: 10.1080/1065657X.(2018).1496045
• Rekadwad, B., J.M. González. (2018). Multidisciplinary involvement and potential of thermophiles. Folia Microbiologica 64(3): 389-406.
• González, J.M. (2018). Molecular tunnels in enzymes and thermophily. A case study on the relationship to growth temperature. MDPI Microorganisms 6: 109. doi:10.3390/microorganisms6040109
• Rekadwad, B.N., González, J.M. (2017). Correcting names of bacteria deposited in national microbial repositories: an analysed sequence data necessary for taxonomic re-categorization of misclassified bacteria – one example, genus Lysinibacillus. Data in Brief 13: 761-778. doi:
• Dominguez, M.T., E. Gutierrez, B.R. Gonzalez-Dominguez, J.M. Avila, C. Ramo, J.M. González, L.V. Garcia. (2017). Impacts of protected colonial birds on soil microbial communities: when protection leads to degradation. Soil Biology and Biochemistry 105: 59-70.
• Cuecas, A., W. Kanoksilapatham, González, J.M. (2017). Evidence of horizontal gene transfer by transposase gene analyses in Fervidobacterium species. PLoS One 12(4): e0173961. doi:
• Santana, M.M., J.M. González, C. Cruz. (2017). Nitric oxide accumulation: the evolutionary trigger for phytopathogenesis. Frontiers in Microbiology 8:1947. doi: 10.3389/fmicb.2017.01947
• Cardozo, F.A., J.M. González, V.A. Feitosa, A. Pessoa, I.N.G. Rivera. (2017). Bioconversion of α-chitin into N-acetyl-glucosamine using chitinases produced by marine-derived Aeromonas caviae isolates. World Journal Microbiology and Biotechnology 33: 201-211.
• Santana, M.M., J.M. González, M.I. Clara. (2016). Inferring pathways leading to organic-sulfur mineralization in the Bacillales. Critical Reviews in Microbiology 42(1): 31-45. DOI:10.3109/1040841X.2013.877869
• Gaspar, H., R. Ferreira, J.M. González, M.I. da Clara, M.M. Santana. (2016). Influence of temperature and copper on Oxalobacteraceae in soil enrichments. Current Microbiology 72: 370-376. doi: 10.1007/s00284-015-0960-1
• Villahermosa, D., A. Corzo, E. Garcia-Robledo, J.M. González, S. Papaspyrou. (2016). Kinetics of indigenous nitrate reducing sulfide oxidizing activity in microaerophilic wastewater biofilms. Plos One 11(2): e0149096. doi: 10.1371/journal.pone.0149096.
• Cuecas, A., J. Cruces, J.F. Galisteo-López, X. Peng, J.M. González. (2016). Cellular viscosity in prokaryotes and thermal stability of low-molecular weight biomolecules. Biophysical Journal 111(4): 875-882. doi: 10.1016/j.bpj.2016.07.024.
• Kanoksilapatham, W., P. Pasomsup, P. Keawram, A. Cuecas, M.C. Portillo, J.M. González. (2016). Fervidobacterium thailandense sp. nov., a novel e extreme thermophilic bacterium isolated from a hot spring in Northern Thailand. International Journal of Systematic and Evolutionary Microbiology 66: 5023-5027. doi: 10.1099/ijsem.0.001463.
• Rekadwad, B., J.M. González, N. Chandrahasya. (2016). Genomic analysis of a marine bacterium: A bioinformatics for comparison, evaluation and interpretation of DNA sequences. BioMed Research International, Microbiology (2016): 7215379. doi: 10.1155/2016/7215379.
• Muñoz, C., F.G. Fermoso, M. Rivas, J.M. González. (2016). Hydrolytic enzyme activity enhanced by Barium supplementation. AIMS Microbiology 2(4): 402-411. doi: 10.3934/microbiol.2016.4.402.
• González, J.M., M.C. Portillo, M. Piñeiro-Vidal. (2015). Latitude-dependent underestimation of microbial extracellular enzyme activity in soils. International Journal of Environmental Science and Technology 12(7): 2427-2434. doi:10.1007/s13762-014-0635-7.
• Lucena-Padrós, H., J.M. González, B. Caballero-Guerrero, J.L. Ruiz-Barba, A. Maldonado-Barrágan. (2015). Vibrio olivae sp. nov., isolated from Spanish-style green-olive fermentations. International Journal of Systematic and Evolutionary Microbiology 65: 1895-1901.
• Reina, M., M.C. Portillo, L. Serrano, E.C.H.E.T. Lucassen, J.G.M. Roelofs, A. Romero, J.M. González. (2015). The interplay of hydrological, chemical and microbial processes in the formation of floating surface iron-rich films in aquatic environments at a circumneutral pH. Limnetica 34: 365-380.
• Kanoksilapatham, W., P. Keawram, J.M. González, F. Robb. (2015). Isolation, characterization and survival strategies of Thermotoga sp. strain PD524, a hyperthermophile from a hot spring in Northern Thailand. Extremophiles 19(4): 853-861 DOI: 10.1007/s00792-015-0761-2
• Ferreira, R., H. Gaspar, J.M. González, M.I. Clara, M.M. Santana. (2015). Copper and temperature modify microbial communities, ammonium and sulfate release in soil. Journal of Plant Nutrition and Soil Science 178: 953-962. DOI: 10.1002/jpln.201500318
• Santana, M.M., J.M. González. (2015). High temperature microbial activity in upper soil layers. FEMS Microbiology Letters 362: 1-4. DOI:
• Sant’Anna, F.H., T. Sokolova, A.V. Lebendinsky, F.T. Robb, J.M. González. (2015). Analysis of three genomes within the thermophilic hydrogenogenic bacterial species Caldanaerobacter subterraneus with a focus on carbon monoxide dehydrogenase evolution and hydrolase diversity. BMC Genomics 16: 757. doi:10.1186/s12864-015-1955-9
• Lucena-Padrós, H., J.M. González, B. Caballero-Guerrero, J.L. Ruiz-Barba, A. Maldonado-Barragán. (2014). Enterococcus olivae sp. nov., isolated from Spanish-style green olive fermentation. International Journal of Systematic and Evolutionary Microbiology 64: 2534-2539.
• Cuecas, A., M.C. Portillo, W. Kanoksilapatham, J.M. González. (2014). Bacterial distribution along a 50ºC temperature gradient reveals a parcelled out hot spring environment. Microbial Ecology 68: 729-739. doi: 10.1093/femsle/fnv182
• Gazulla, M.F., E. Sánchez, J.M. González, M. Orduña. (2014). Biodeterioration behaviour in different colour roofing tiles (red and straw coloured). Comportamiento de tejas de diferente color (rojo y paja) frente al biodeterioro. Bolletin de la Sociedad Española de Cerámica y Vidrio 53(5): 227-234.
• González, I. Clara. (2013). Characterization of new soil thermophilic bacteria potentially involved in soil fertilization. Journal of Plant Nutrition and Soil Science 176: 47-56. doi: 10.1002/jpln.201100382
• Sánchez-Porro, C., R. de la Haba, N. Cruz-Hernández, J.M. González, C. Reyes-Guirao, L. Navarro-Sampedro, M. Carballo, A. Ventosa. (2013). Draft Genome of the Marine Gammaproteobacterium Halomonas titanicae. Journal of Bacteriology. Genome Announcements (genomeA00083-13) 1(2): e00218-12.
• Villahermosa, D., A. Corzo, J.M. González, M.C. Portillo, E. García-Robledo, S. Papaspyrou. (2013). Reduction of net sulfide production rate by nitrate in wastewater bioreactors. Kinetics and changes in the microbial community. Water, Air and Soil Pollution 224: 1738-1753.
• Rincón, B., M.C. Portillo, J.M. González, R. Borja. (2013). Microbial communities analyses in a two-stage anaerobic digestion process treating two-phase olive mill solid residue. International Journal of Environmental Science and Technology 10: 635-644.
• Sanchez-Moral, S., M. C. Portillo, I. Janices, S. Cuezva, A. Fernández-Cortés, J. C. Cañaveras, J.M. González. (2012). The role of microorganisms in the formation of calcitic moonmilk deposits and speleothems in Altamira Cave. Geomorphology 139-140: 285-292.
• Portillo, M. C., M. Santana, J. M. González. (2012). Presence and potencial role of thermophilic bacteria in temperate terrestrial environments. Naturwissenschaften 99: 43-53.
• Zimmermann, J., M.C. Portillo, L. Serrano, J.M. González. (2012). Acidobacteria in freshwater ponds at Doñana National Park, Spain. Microbial Ecology 63: 844-855.
• González, J.M., M.C. Portillo, P. Belda-Ferre, A. Mira. (2012). Amplification by PCR artificially reduces the proportion of the rare biosphere in microbial communities. Plos One 7 (1): e29973. doi: 10.1371/journal.pone.0029973.
• Kanoksilapatham, W., P. Pasomsup, M.C. Portillo, P. Keawram, J.M. González. (2012). Identification and characterization of a freshwater Pyrococcus sp. strain PK 5017 and identification of pfu-like IS elements in Thermococcus sibiricus MM 739. International Journal of Biology 4: 11-22.
• Santana, M., M.C. Portillo, J.M. González. (2012). Mutualistic growth of the sulfate-reducer Desulfovibrio vulgaris Hildenborough with different carbohydrates. Microbiology 81(6): 663-668.
1 Protocol published
One Plate-double Nutrient Endospore Activation Method
Authors:  Bhagwan Narayan Rekadwad, Juan M. Gonzalez and Chandrahasya N. Khobragade, date: 01/05/2020, view: 91, Q&A: 0
This paper presents a simple, swift, improved and inexpensive one plate-double nutrient endospore activation method (ODNEAM) for activation of thermophilic endospore (thermospore). Revival and cultivation of dormant bacteria experiencing ...
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