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Bio-protocol Editor

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Microbiology

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10 Protocols published

RNA Preparation for Microarray Experiments

Author: Bio-protocol Editor, date: 01/20/2011, view: 13126, Q&A: 0

This protocol describes a simple and relatively general method to extract total RNA from a yeast culture using the Qiagen RNeasy kit. In it, total RNA is treated with DNase I and cleaned up to be suitable for microarray experiments. Therefore, this ...

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Yeast Vacuole Staining with FM4-64

Author: Bio-protocol Editor, date: 01/05/2011, view: 23595, Q&A: 2

The lipophilic probe, FM 4-64 does not fluoresce much in water but fluoresces strongly after binding to the outer plasma membrane, providing clear and distinguishable plasma membrane staining. The binding is rapid and reversible. In this protocol ...

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Abaya Prakash

Heidelberg University