Grisel Cruz-Becerra Section of Molecular Biology, University of California San Diego, La Jolla
1 protocol

James Takuro Kadonaga
  • Faculty, University of California San Diego La Jolla
Research focus
  • Molecular biology
  • Regulation of transcription by RNA polymerase II
    Chromatin dynamics
  • 1 Author merit
1 Protocol published
Reconstitution of Chromatin by Stepwise Salt Dialysis
Authors:  Grisel Cruz-Becerra and James T. Kadonaga, date: 04/05/2021, view: 1406, Q&A: 0

Chromatin, rather than plain DNA, is the natural substrate of the molecular machines that mediate DNA-directed processes in the nucleus. Chromatin can be reconstituted in vitro by using different methodologies. The salt dialysis method yields

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