Kossay Zaoui Université de Montréal Hospital Centre Research Centre (CRCHUM)
1 protocol

Raquel Brandao Haga Max Planck Institute of Biochemistry
1 protocol

xiang luo Guangdong Medicine University
2 protocols

Aleksei A Tikhonov
  • Research associate, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Research focus
  • Cancer biology
  • cancer biomarkers, clinical chemistry, translational research, microarray, antibodies, tumor immunology, glycomics


Ph.D., EIMB RAS, 2021

Lab information

Laboratory of biological microchips



• Tikhonov, A., Savvateeva, E., Chernichenko, M., Maslennikov, V., Sidorov, D., Rubina, A., and Kushlinskii, N. Analysis of Anti-Glycan IgG and IgM Antibodies in Colorectal Cancer. Bulletin of experimental biology and medicine, 2019, 166(4), 489-493.
• Tikhonov, A., Chernichenko, M., Butvilovskaya, V., Sidorov, D., and Rubina, A. IgM anti-glycan antibodies in sera of colorectal cancer patients. Annals of Oncology, 2018, 29(suppl_6).
• Tikhonov, A., Butvilovskaya, V., Chernichenko, M., Savvateeva, E., Feyzkhanova, G., Lysov, Y., Sidorov, D., and Rubina, A. Diagnostic value of anti-glycan antibodies in patients with colorectal cancer. Annals of Oncology, 2017, 28(suppl_7).
• Savvateeva, E., Tikhonov, A., Butvilovskaya, V., Tsybulskaya, М., and Rubina, A. Exosomal surface protein markers in diagnosis of colorectal cancer. Molecular Biology, 2017, 51(5), 659-665.
• Butvilovskaya, V., Tikhonov, A., Savvateeva, E., Ragimov, A., Salimov, E., Voloshin, S., Sidorov, D., Chernichenko, M., Polyakov, A., Filushin, M., Tsybulskaya, М., and Rubina, A. Hydrogel microchip as a tool for studying exosomes in human serum. Molecular Biology, 2017, 51(5), 712-717.
• Feyzkhanova, G., Voloshin, S., Smoldovskaya, O., Arefieva, A., Filippova, M., Barsky, V., Pavlushkina, L., Butvilovskaya, V., Tikhonov, A., Reznikov, Yu., and Rubina, A. Development of a microarray-based method for allergen-specific IgE and IgG4 detection. Clinical Proteomics, 2017, 14(1), 1.
• Arefieva, A., Smoldovskaya, O., Tikhonov, A., and Rubina, A. Allergy and autoimmunity: Molecular diagnostics, therapy, and presumable pathogenesis. Molecular Biology, 2017, 51(2), 194-204.
• Tikhonov, A., Tsybulskaya, М., Butvilovskaya, V., Savvateeva, E., Belousov, P., Kuprash, D., Solopova, O., Chernichenko, M., Filushin, M., and Rubina, A. Differential quantification of SCCA1 and SCCA2 cancer antigens using a hydrogel biochip. Analytical Methods, 2016, 8(44), 7920-7928.
• Butvilovskaya, V., Tsybulskaya, M., Tikhonov, A., Talibov, V., Belousov, P., Sazykin, A., Schwartz, A., Surzhikov, S., Stomakhin, A., Solopova, O., and Rubina, A. Preparation of recombinant serpins B3 and B4 and investigation of their specific interactions with antibodies using hydrogel-based microarrays. Molecular Biology, 2015, 49(5), 705-713.
• Tikhonov, A., Vasil’ev, A., Chirskaya, M., Struchkova, M., Merkulova, N., and Zlotin, S. One-pot synthesis of substituted styrenes from vicinal dibromoalkanes and arylboronic acids. Russian Chemical Bulletin, 2007, 56(1), 122-129.


• Butvilovskaya, Popletaeva, Chechetkin, Rubina, Savvateeva, Tsybulskaya, Voloshin and Tikhonov. The method of diagnosis/screening of colorectal cancer, based on the simultaneous quantification of tumor markers such as proteins, antibodies to glycans, immunoglobulins G, A and, M in human blood on a biological microchip. RU Patent No 2625018, December 16, 2015.
• Butvilovskaya, Savvateeva, Tikhonov, Voloshin, Tsybulskaya, and Rubina. Biological microchip for detection of tumor exosomes in human serum/plasma for the diagnosis of colorectal cancer. RU Patent No 2682721, November 17, 2016.
2 Protocols reviewed
Preparation of an Orthotopic, Syngeneic Model of Lung Adenocarcinoma and the Testing of the Antitumor Efficacy of Poly(2-oxazoline) Formulation of Chemo-and Immunotherapeutic Agents

Tumor xenograft models developed by transplanting human tissues or cells into immune-deficient mice are widely used to study human cancer response to drug candidates. However, immune-deficient mice are unfit for investigating the effect of

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In vitro Crosslinking Reactions and Substrate Incorporation Assays for The Identification of Transglutaminase-2 Protein Substrates
Authors:  William L. Willis, Abigail Foster, Caitlin Henry, Lai Chu Wu and Wael Jarjour, date: 06/20/2020, view: 1523, Q&A: 0
Transglutaminase (TG2) catalyzes protein crosslinking between glutamyl and lysyl residues. Catalytic activity occurs via a transamidation mechanism resulting in the formation of isopeptide bonds. Since TG2-mediated transamidation is of mechanistic ...
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