Kay-Hooi Khoo Institute of Biological Chemistry, Academia Sinica, Taiwan
2 protocols

Chu-Wen Cheng 1 protocol

Sergei Snovida 1 protocol

Shin-Yi YU
  • Post-doctorial fellow, Unversité Lille/UMR-995-Unité de Glycobiologie Structurale et Fonctionnelle, France
Research focus
  • mass spectrometry
  • 2 Author merit


PhD, National Taiwan University/Institute of Biochemical Science, Taiwan, 2011


• Yamakawa, N., Vanbeselaere, J., Chang, L. Y., Yu, S. Y., Ducrocq, L., Harduin-Lepers, A., Kurata, J., Aoki-Kinoshita, K. F., Sato, C., Khoo, K. H., Kitajima, K. and Guerardel, Y. (2018). Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns. Nat Commun 9(1): 4647.
• Yu, S. Y., Hsiao, C. T., Izawa, M., Yusa, A., Ishida, H., Nakamura, S., Yagi, H., Kannagi, R. and Khoo, K. H. (2018). Distinct substrate specificities of human GlcNAc-6-sulfotransferases revealed by mass spectrometry-based sulfoglycomic analysis. J Biol Chem 293(39): 15163-15177.
• Dulary, E., Yu, S. Y., Houdou, M., de Bettignies, G., Decool, V., Potelle, S., Duvet, S., Krzewinski-Recchi, M. A., Garat, A., Matthijs, G., Guerardel, Y. and Foulquier, F. (2018). Investigating the function of Gdt1p in yeast Golgi glycosylation. Biochim Biophys Acta Gen Subj 1862(3): 394-402.
• Chen, J. Y., Huang, H. H., Yu, S. Y., Wu, S. J., Kannagi, R. and Khoo, K. H. (2018). Concerted mass spectrometry-based glycomic approach for precision mapping of sulfo sialylated N-glycans on human peripheral blood mononuclear cells and lymphocytes. Glycobiology 28(1): 9-20.
• Kannagi, R., Sakuma, K., Cai, B. H., Yu, S. Y. (2015) Tumor-Associated Glycans and Their Functional Roles in the Multistep Process of Human Cancer Progression. In: Suzuki T., Ohtsubo Shin-Yi YU/ Form No. 121265/ Section No. 16/02, 16/03 K., Taniguchi N. (eds) Sugar Chains. Springer, Tokyo, 139-158.
• Yu, S. Y., Chang, L. Y., Cheng, C. W., Chou, C. C., Fukuda, M. N. and Khoo, K. H. (2013). Priming mass spectrometry-based sulfoglycomic mapping for identification of terminal sulfated lacdiNAc glycotope. Glycoconj J 30(2): 183-194.
• Patnode, M. L., Yu, S. Y., Cheng, C. W., Ho, M. Y., Tegesjo, L., Sakuma, K., Uchimura, K., Khoo, K. H., Kannagi, R. and Rosen, S. D. (2013). KSGal6ST generates galactose-6-O-sulfate in high endothelial venules but does not contribute to L-selectin-dependent lymphocyte homing. Glycobiology 23(3): 381-394.
• Shibata, T. K., Matsumura, F., Wang, P., Yu, S., Chou, C. C., Khoo, K. H., Kitayama, K., Akama, T. O., Sugihara, K., Kanayama, N., Kojima-Aikawa, K., Seeberger, P. H., Fukuda, M., Suzuki, A., Aoki, D. and Fukuda, M. N. (2012). Identification of mono- and disulfated N-acetyl-lactosaminyl Oligosaccharide structures as epitopes specifically recognized by humanized monoclonal antibody HMOCC-1 raised against ovarian cancer. J Biol Chem 287(9): 6592-6602.
• Kobayashi, M., Mitoma, J., Hoshino, H., Yu, S. Y., Shimojo, Y., Suzawa, K., Khoo, K. H., Fukuda, M. and Nakayama, J. (2011). Prominent expression of sialyl Lewis X-capped core 2-branched O-glycans on high endothelial venule-like vessels in gastric MALT lymphoma. J Pathol 224(1): 67-77.
• Yagi, H., Yamamoto, M., Yu, S. Y., Takahashi, N., Khoo, K. H., Lee, Y. C. and Kato, K. (2010). N-Glycosylation profiling of turtle egg yolk: expression of galabiose structure. Carbohydr Res 345(3): 442-448.
• Wada, Y., Dell, A., Haslam, S. M., Tissot, B., Canis, K., Azadi, P., Backstrom, M., Costello, C. E., Hansson, G. C., Hiki, Y., Ishihara, M., Ito, H., Kakehi, K., Karlsson, N., Hayes, C. E., Kato, K., Kawasaki, N., Khoo, K. H., Kobayashi, K., Kolarich, D., Kondo, A., Lebrilla, C., Nakano, M., Narimatsu, H., Novak, J., Novotny, M. V., Ohno, E., Packer, N. H., Palaima, E., Renfrow, M. B., Tajiri, M., Thomsson, K. A., Yagi, H., Yu, S. Y. and Taniguchi, N. (2010). Comparison of methods for profiling O-glycosylation: Human Proteome Organisation Human Disease Glycomics/Proteome Initiative multi-institutional study of IgA1. Mol Cell Proteomics 9(4): 719-727.
• Lee, S. H., Yu, S. Y., Nakayama, J., Khoo, K. H., Stone, E. L., Fukuda, M. N., Marth, J. D. and Fukuda, M. (2010). Core2 O-glycan structure is essential for the cell surface expression of sucrase isomaltase and dipeptidyl peptidase-IV during intestinal cell differentiation. J Biol Chem 285(48): 37683-37692.
• Khoo, K. H. and Yu, S. Y. (2010). Mass spectrometric analysis of sulfated N- and O-glycans. Methods Enzymol 478: 3-26.
• Yu, S. Y., Yang, Z., Khoo, K. H. and Wu, A. M. (2009). Identification of blood group A/A-Leb/y and B/B-Leb/y active glycotopes co-expressed on the O-glycans isolated from two distinct human ovarian cyst fluids. Proteomics 9(13): 3445-3462.
• Yu, S. Y., Wu, S. W., Hsiao, H. H. and Khoo, K. H. (2009). Enabling techniques and strategic workflow for sulfoglycomics based on mass spectrometry mapping and sequencing of permethylated sulfated glycans. Glycobiology 19(10): 1136-1149.
• Lee, S. H., Hatakeyama, S., Yu, S. Y., Bao, X., Ohyama, C., Khoo, K. H., Fukuda, M. N. and Fukuda, M. (2009). Core3 O-glycan synthase suppresses tumor formation and metastasis of prostate carcinoma PC3 and LNCaP cells through down-regulation of alpha2beta1 integrin complex. J Biol Chem 284(25): 17157-17169.
• Yu, S. Y., Khoo, K. H., Yang, Z., Herp, A. and Wu, A. M. (2008). Glycomic mapping of O- and N-linked glycans from major rat sublingual mucin. Glycoconj J 25(3): 199-212.
• Yagi, H., Yasukawa, N., Yu, S. Y., Guo, C. T., Takahashi, N., Takahashi, T., Bukawa, W., Suzuki, T., Khoo, K. H., Suzuki, Y. and Kato, K. (2008). The expression of sialylated high-antennary N-glycans in edible bird's nest. Carbohydr Res 343(8): 1373-1377.
• Garenaux, E., Yu, S. Y., Florea, D., Strecker, G., Khoo, K. H. and Guerardel, Y. (2008). A single step method for purification of sulfated oligosaccharides. Glycoconj J 25(9): 903-915.
• Fan, Y. Y., Yu, S. Y., Ito, H., Kameyama, A., Sato, T., Lin, C. H., Yu, L. C., Narimatsu, H. and Khoo, K. H. (2008). Identification of further elongation and branching of dimeric type 1 chain on lactosylceramides from colonic adenocarcinoma by tandem mass spectrometry sequencing analyses. J Biol Chem 283(24): 16455-16468.
• Wu, A. M., Khoo, K. H., Yu, S. Y., Yang, Z., Kannagi, R. and Watkins, W. M. (2007). Glycomic mapping of pseudomucinous human ovarian cyst glycoproteins: identification of Lewis and sialyl Lewis glycotopes. Proteomics 7(20): 3699-3717.
• Sutton-Smith, M., Wong, N. K., Khoo, K. H., Wu, S. W., Yu, S. Y., Patankar, M. S., Easton, R., Lattanzio, F. A., Morris, H. R., Dell, A. and Clark, G. F. (2007). Analysis of protein-linked glycosylation in a sperm-somatic cell adhesion system. Glycobiology 17(6): 553-567.
• Mitoma, J., Bao, X., Petryanik, B., Schaerli, P., Gauguet, J. M., Yu, S. Y., Kawashima, H., Saito, H., Ohtsubo, K., Marth, J. D., Khoo, K. H., von Andrian, U. H., Lowe, J. B. and Fukuda, M. (2007). Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment. Nat Immunol 8(4): 409-418.
• Yu, S. Y., Wu, S. W. and Khoo, K. H. (2006). Distinctive characteristics of MALDI-Q/TOF and TOF/TOF tandem mass spectrometry for sequencing of permethylated complex type N-glycans. Glycoconj J 23(5-6): 355-369.
2 Protocols published
Negative Ion Mode nanoLC-ESI-MS/MS Analyses of Permethylated Sulfated Glycans
Authors:  Shin-Yi Yu, Chu-Wen Cheng and Kay-Hooi Khoo, date: 05/20/2020, view: 1920, Q&A: 0
We have developed enabling techniques for sulfoglycomics based on MALDI-MS mapping and MS/MS sequencing of permethylated sulfated glycans. We then extended further the analytical workflow to C18 reverse phase (RP)-nanoLC-nanoESI-MS/MS analyses of ...
Permethylation and Microfractionation of Sulfated Glycans for MS Analysis
Authors:  Shin-Yi Yu, Sergei Snovida and Kay-Hooi Khoo, date: 05/20/2020, view: 1976, Q&A: 0
Sulfated glycans are barely detectable in routine mass spectrometry (MS)-based glycomic analysis due to ion suppression by the significantly more abundant neutral glycans in the positive ion mode, and sialylated non-sulfated glycans in the negative ...
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