James Clegg Simons Initiative for the Developing Brain & Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh
1 protocol

James Clegg 1 protocol

Thomas Pratt
  • Faculty, University of Edinburgh Edinburgh
  • 2 Author merit

Education

PhD, The University of Edinburgh, 2001

Lab information

Thomas Pratt Lab
https://www.ed.ac.uk/discovery-brain-sciences/our-staff/research-groups/tom-pratt

2 Protocols published
Ligand and Carbohydrate Engagement (LACE) Assay and Fluorescence Quantification on Murine Neural Tissue
Authors:  James M. Clegg and Thomas Pratt, date: 03/20/2021, view: 590, Q&A: 0

The interaction between cell surface heparan sulphate and diffusible ligands such as FGFs is of vital importance for downstream signaling, however, there are few techniques that can be used to investigate this binding event. The ligand and

...
Organotypic Slice Culture of the Embryonic Mouse Brain
Authors:  James M. Clegg and Thomas Pratt, date: 07/05/2020, view: 1396, Q&A: 0
Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays ...
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