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A peer-reviewed protocol journal. No publication fee; no access fee.
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Kohei Suzuki
Internal Medicine, Division of Digestive and Liver DIseases University of Texas Southwestern Medical Center
1 protocol
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Ryuichi Okamoto
Professor, Department of Gastroenterology and Hepatology Tokyo Medical and Dental University (TMDU)
Research focus
Stem cell
Contributions
1
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Education
Graduate School, Tokyo Medical and Dental University (TMDU)
Publications
https://scholar.google.co.jp/citations?user=BszbhZMAAAAJ&hl=ja
1 Protocol published
Fluidigm Based Single-cell Gene Expression Library Preparation from Patient-derived Small Intestinal Organoids
Authors:
Kohei Suzuki
and
Ryuichi Okamoto
,
date:
10/05/2020,
view:
1132,
Q&A:
0
In this protocol, we describe our methods to isolate crypts from patients' biopsy samples and to culture human intestinal stem cells as it’s called “organoid.” Beyond that, we describe how to dissociate organoids cells into single cells for ...
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