Ryuichi Okamoto —BIO-PROTOCOL

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Kohei Suzuki Internal Medicine, Division of Digestive and Liver DIseases University of Texas Southwestern Medical Center
1 protocol

Ryuichi Okamoto

Professor, Department of Gastroenterology and Hepatology Tokyo Medical and Dental University (TMDU)

Research focus

Stem cell

1 Author merit
Reviewer merit
Editor merit

Education

Graduate School, Graduate School

Publications

https://scholar.google.co.jp/citations?user=BszbhZMAAAAJ&hl=ja

1 Protocol published

Fluidigm Based Single-cell Gene Expression Library Preparation from Patient-derived Small Intestinal Organoids

Authors: Kohei Suzuki and Ryuichi Okamoto, date: 10/05/2020, view: 547, Q&A: 0

In this protocol, we describe our methods to isolate crypts from patients' biopsy samples and to culture human intestinal stem cells as it’s called “organoid.” Beyond that, we describe how to dissociate organoids cells into single cells for ...

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