Kohei Suzuki Internal Medicine, Division of Digestive and Liver DIseases University of Texas Southwestern Medical Center
1 protocol

Ryuichi Okamoto
  • Professor, Department of Gastroenterology and Hepatology Tokyo Medical and Dental University (TMDU)
Research focus
  • Stem cell
  • 1 Author merit


Graduate School, Tokyo Medical and Dental University (TMDU)


1 Protocol published
Fluidigm Based Single-cell Gene Expression Library Preparation from Patient-derived Small Intestinal Organoids
Authors:  Kohei Suzuki and Ryuichi Okamoto, date: 10/05/2020, view: 1132, Q&A: 0
In this protocol, we describe our methods to isolate crypts from patients' biopsy samples and to culture human intestinal stem cells as it’s called “organoid.” Beyond that, we describe how to dissociate organoids cells into single cells for ...
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