Diego Acosta-Alvear —BIO-PROTOCOL

Home About Us

Archive

Peter Walter 1 protocol

Jirka Peschek 1 protocol

G. Karagöz Max Perutz Labs Vienna, Medical University of Vienna, Vienna
1 protocol

Alessandro Didonna University of California, San Francisco
43 protocols

Maria Dermit Maria Dermit
1 protocol

Reviewer

Diego Acosta-Alvear

Faculty, Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara

Research focus

Molecular biology
cellular stress responses, unfolded protein response, RNA biology

1 Author merit
Reviewer merit
Editor merit

Education

PhD, New York University, 2008

1 Protocol published

In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay

Authors: G. Elif Karagöz, Jirka Peschek, Peter Walter and Diego Acosta-Alvear, date: 07/20/2019, view: 3878, Q&A: 0

The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube. Here we describe a protocol for the in vitro cleavage and analysis of ...

More >

1 Protocol reviewed

Novel Protein-oligonucleotide Conjugation Method Involving a High-affinity Capture HaloTag

Author: Junshi Yazaki, date: 09/20/2020, view: 1788, Q&A: 0

Highly sensitive quantitative protein profiling can play a key role in the early diagnosis of diseases, such as autoimmune diseases and cancer. We developed a modified protein-oligonucleotide conjugation method termed HaloTag-mediated barcoding, for ...

More >