Chu-Wen Cheng 1 protocol

Shin-Yi YU Unversité Lille/UMR-995-Unité de Glycobiologie Structurale et Fonctionnelle, France
2 protocols

Sergei Snovida 1 protocol

Kay-Hooi Khoo
  • Faculty, Institute of Biological Chemistry, Academia Sinica, Taiwan
Research focus
  • 2 Author merit
2 Protocols published
Negative Ion Mode nanoLC-ESI-MS/MS Analyses of Permethylated Sulfated Glycans
Authors:  Shin-Yi Yu, Chu-Wen Cheng and Kay-Hooi Khoo, date: 05/20/2020, view: 1826, Q&A: 0
We have developed enabling techniques for sulfoglycomics based on MALDI-MS mapping and MS/MS sequencing of permethylated sulfated glycans. We then extended further the analytical workflow to C18 reverse phase (RP)-nanoLC-nanoESI-MS/MS analyses of ...
Permethylation and Microfractionation of Sulfated Glycans for MS Analysis
Authors:  Shin-Yi Yu, Sergei Snovida and Kay-Hooi Khoo, date: 05/20/2020, view: 1879, Q&A: 0
Sulfated glycans are barely detectable in routine mass spectrometry (MS)-based glycomic analysis due to ion suppression by the significantly more abundant neutral glycans in the positive ion mode, and sialylated non-sulfated glycans in the negative ...
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