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Chu-Wen Cheng
1 protocol
Shin-Yi YU
Unversité Lille/UMR-995-Unité de Glycobiologie Structurale et Fonctionnelle, France
2 protocols
Sergei Snovida
1 protocol
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Kay-Hooi Khoo
Faculty, Institute of Biological Chemistry, Academia Sinica, Taiwan
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2 Protocols published
Negative Ion Mode nanoLC-ESI-MS/MS Analyses of Permethylated Sulfated Glycans
Authors:
Shin-Yi Yu
,
Chu-Wen Cheng
and
Kay-Hooi Khoo
,
date:
05/20/2020,
view:
613,
Q&A:
0
We have developed enabling techniques for sulfoglycomics based on MALDI-MS mapping and MS/MS sequencing of permethylated sulfated glycans. We then extended further the analytical workflow to C18 reverse phase (RP)-nanoLC-nanoESI-MS/MS analyses of ...
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Permethylation and Microfractionation of Sulfated Glycans for MS Analysis
Authors:
Shin-Yi Yu
,
Sergei Snovida
and
Kay-Hooi Khoo
,
date:
05/20/2020,
view:
685,
Q&A:
0
Sulfated glycans are barely detectable in routine mass spectrometry (MS)-based glycomic analysis due to ion suppression by the significantly more abundant neutral glycans in the positive ion mode, and sialylated non-sulfated glycans in the negative ...
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