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Tomoyuki Yamaguchi
Division of Stem Cell Therapy, The Universiyt of Tokyo, Institute of Medical Science
1 protocol
Hiromitsu Nakauchi
1 protocol
Mariko Kasai
1 protocol
Hideyuki Sato
1 protocol
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Eiji Mizutani
Research focus
Stem cell
Contributions
1
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1 Protocol published
CRISPR/Cas9 + AAV-mediated Intra-embryonic Gene Knocking in Mice
Authors:
Naoaki Mizuno
,
Eiji Mizutani
,
Hideyuki Sato
,
Mariko Kasai
,
Hiromitsu Nakauchi
and
Tomoyuki Yamaguchi
,
date:
07/05/2019,
view:
2713,
Q&A:
0
Intra-embryo genome editing by CRISPR/Cas9 has enabled rapid generation of gene knockout animals. However, large fragment knock-in directly into embryos’ genome is still difficult, especially without microinjection of donor DNA. Viral vectors are ...
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