Maria Agallou Department of Microbiology, Hellenic Pasteur Institute, Athens, Greece
1 protocol

Evdokia Karagouni
  • Research Director, Department of Microbiology, Hellenic Pasteur Institute, Athens, Greece
Research focus
  • -
  • 1 Author merit


Ph.D., School of Biology, Aristotle University of Thessaloniki, Greece, 1989



· Agallou, M., Pantazi, E., Tsiftsaki, E., Toubanaki, D.K., Gaitanaki, C., Smirlis, D. and Karagouni, E. (2018) Induction of protective cellular immune responses against experimental visceral leishmaniasis mediated by dendritic cells pulsed with the N-terminal domain of Leishmania infantum elongation factor-2 and CpG oligodeoxynucleotides. Molecular Immunology; 103: 7-20.
· Toubanaki DK, Karagouni E. (2018) Towards a Dual Lateral Flow Nanobiosensor for Simultaneous Detection of Virus Genotype-Specific PCR Products. J Anal Methods Chem. 2018 Feb 20;2018:7691014. doi: 10.1155/2018/7691014. eCollection 2018.

2013 – 2017 Period

· Karagouni, E., O. Kammona, M. Margaroni, K. Kotti, V. Karageorgiou, C. Gaitanaki and C. Kiparissides (2013). Uptake of BSA-FITC loaded PLGA nanoparticles by bone marrow-derived dendritic cells induces maturation but not IL-12 or IL-10 production. Nanoscience & Nanotechnology Letters 5(4), 498-504.
· Tzouvelekis, L.S., V. Miriagou, S.D. Kotsakis, E. Athanasiou, E. Karagouni, E. Tzelepi and G.L. Daikos (2013). KPC-producing, multi-resistant Klebsiella pneumonie sequence type 258 as a typical opportunistic pathogen. Antimicrobial Agents and Chemotherapy 57(10), 5144-6.
· Rigos, G., E. Karagouni, I. Kyriazis, E. Athanasiou, K. Grigorakis, E. Kotou and P. Katharios (2013). In vitro and in vivo evaluation of quinine in gilthead sea bream, Sparus aurata naturally infected with the ciliate Cryptocaryon irritans. Aquaculture 416-417, 185-91.
· Agallou, M., E. Dotsika, S. Frydas, and E. Karagouni (2014). Toll-like receptor 4 promotes control of Leishmania infantum infection through inducement of leishmanicidal activity in host macrophages: role of mitogen activated kinases. Journal of Biological Regulators & Homeostatic Agents 28(1), 41-52.
· Agallou, M., E. Dotsika and E. Karagouni (2014). Low CD40 expression levels in Leishmania infantum-infected bone marrow dendritic cells evoke regulatory responses by down-regulating interleukin-12 production: Role of ERK1/2. European Journal of Inflammation 12(2), 315-328.
· Agallou, M., E. Athanasiou, O. Koutsoni, E. Dotsika and E. Karagouni (2014). Experimental validation of multi-epitope peptides including promising MHC class I- and II-restricted epitopes of four known Leishmania infantum proteins. Frontiers in Immunology 5, 268.
· Giannenas, I., I. Karamaligas, M. Margaroni, I. Pappas, E. Mayer, P. Encarnação and E. Karagouni (2015). Effect of dietary incorporation of a multi-strain probiotic on growth performance and health status in rainbow trout (Oncorhynchus mykiss). Fish Physiology & Biochemistry 41(1), 119-28.
· Toubanaki, D.K., M. Margaroni and E. Karagouni (2015). Nanoparticle-based lateral flow biosensor for visual detection of fish nervous necrosis virus amplification products. Molecular & Cellular Probes 29,158-166.
· Pefkianakis, E.K., T.A. Theodossiou, D.K. Toubanaki, E. Karagouni, P. Falaras, K. Papadopoulos, and G.C. Vougioukalakis (2015). A family of potent Ru (II) photosensitizers with enhanced DNA intercalation: bimodal photokillers. Photochemistry and Photobiology 91(5), 1191-1202.
· Toubanaki, D.K., M. Margaroni and E. Karagouni (2015). Development of a novel allele-specific PCR method for rapid assessment of Nervous Necrosis Virus genotypes. Current Microbiology 71,529-539.
· Agallou, M., E. Athanasiou, M. Samiotaki, G. Panagiotou and E. Karagouni (2016). Identification of immunoreactive Leishmania infantum protein antigens to asymptomatic dog sera through combined immunoproteomic and bioinformatic analysis. PLOS One 11(2): e0149894.
· Toubanaki, D.K., M. Margaroni and E. Karagouni (2016). Dual enhancement with a nanoparticle-based lateral flow biosensor for the determination of DNA. Analytical Letters 49(7), 1040-1055.
· Margaroni, M., M. Agallou, K. Kontonikola, K. Karidi, O. Kammona, C. Kiparissides, C. Gaitanaki and E. Karagouni (2016). PLGA nanoparticles modified with a TNFa mimicking peptide, soluble Leishmania antigens and MPLA induce T cell priming in vitro via dendritic cell functional differentiation. Journal of Pharmaceutics and Biopharmaceutics105: 18-31.
· Toubanaki, D.K., E. Athanasiou and E. Karagouni (2016). Gold nanoparticle-based lateral flow biosensor for rapid visual detection of Leishmania amplification products. Journal of Microbiological Methods 127, 51–58.
· Toubanaki, D.K. and E. Karagouni (2016). Oligonucleotide-conjugated gold nanoparticles for application on lateral flow biosensors: Evaluation and optimization of low pH and salt-aging conjugation methods. Analytical Letters doi: 10.1080/00032719.2016.1161046.
· Agallou, M., M. Margaroni, E. Athanasiou, D. Toubanaki, K. Kontonikola, O. Kammona, C. Kiparissides and E. Karagouni (2016). Identification of BALB/c immune markers correlated with a partial protection to Leishmania infantum after vaccination with a rationally designed multi-epitope cysteine protease A peptide-based nanovaccine. PLOS Neglected Tropical Diseases 11(1): e0005311.
· Toubanaki DK, E. Karagouni (2017). Genotype-specific real-time PCR combined with high-resolution melting analysis for rapid identification of red-spotted grouper nervous necrosis virus. Archives of Virology 162(8): 2315-2328, doi: 10.1007/s00705-017-3375-4.
· Athanasiou, E., M. Agallou, S. Tastsoglou, O. Kammona, A. Hatzigeorgiou, C. Kiparissides, and E. Karagouni (2017). A poly(lactic-co-glycolic) acid nanovaccine based on chimeric peptides from different Leishmania infantum proteins induces dendritic cells maturation and promotes peptide-specific IFN gamma-producing CD8+ T cells essential for the protection against experimental visceral leishmaniasis. Frontiers in Immunology 8: 684, doi: 10.3389/fimmu.2017.00684.
· Margaroni, M., M. Agallou, E. Athanasiou, C. Kiparissides, C. Gaitanaki and E. Karagouni (2017). Vaccination with poly(D,L-lactide-co-glycolide) nanoparticles loaded with soluble Leishmania antigens and modified with TNF alpha-mimicking peptide ot monophosphoryl lipid A confers protetection against experimental visceral leishmaniasis. International Journal of Nanomedicine 12: 6169-6183. doi: 10.2147/IJN.S141069.

Beginning - 2012

· Hadjipetrou-Kourounakis, L., E. Karagounis, E. Rekka and P. Kourounakis (1989). Immunosuppression by a novel analgesic-opioid agonist. Scand. J. Immunol., 29, 449-458.
· Karagouni, E.E. and L. Hadjipetrou-Kourounakis (1990). Interleukins counteract opioid agonists immunosuppression. Intern. J. Neurosc., 54(1-2), 157-163.
· Karagouni, E.E. and L. Hadjipetrou-Kourounakis (1990). Regulation of isotype immunoglobulin production by adjuvants in vivo. Scand. J. Immunol., 31, 745-754.
· Karagouni, E.E. and L. Hadjipetrou-Kourounakis (1990). Regulation of isotype immunoglobulin and interleukin production by adjuvants in vitro. J. Clin. Lab. Immunol., 33, 29-39.
· Kourounakis, L., A. Gavalas, E.E. Karagouni, D. Litina and P. Kourounakis (1991). Anti-inflammatory and immunomodulating effects of the novel agent -(2-aminoethylamino)-2-butyrothienone (-ABT). 2nd communication: Inhibitory effect on rat adjuvant induced disease. Arzneim. Forsch./ Drug Research 41(I), 5, 537-541.
· Karagouni, E.E. and L. Kourounakis (1991). Anti-inflammatory and immunomodulating effects of the novel agent -(2-aminoethylamino)-2-butyrothienone. 3rd communication: Effect on suppressor cells and cytokines production in normal and adjuvant arthritic rats. Arzneim. Forsch./Drug Research, 41(I), 6, 647-651.
· Haralabidis, S., Frydas, S., Karagouni, E., Himonas, C. (1992). Trichinella-spiralis-Ab3 (IgG, IgM, IgA), IL-6 and TNF levels in murine trichinosis. Int. J. Immunopathol. Pharmacol. 5(3), 173-183.
· Karagouni, E.E., E.N. Dotsika and A. Sklavounou (1994). Alteration in peripheral blood mononuclear cell function and serum cytokines in oral lichen planus. J. Oral Pathol. Med., 23, 28-35.
· Fridas, S., A. Trakatellis, E. Karagouni, E. Dotsika, C. Himonas and P. Conti (1994). 4-Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganate in vivo. Mol. Cell. Biochem. 136, 59-63.
· Karagouni, E., S. Frydas, E. Dotsika, C. Himonas, P. Conti and A. Trakatellis (1995). Inhibition of TNF-α and IL-6, but noy IFN-γ, by 4-deoxypyridoxine (4-PPD) in Trichinella-infected mouse serum. Int. J. Immunopathol. Pharmacol. 8(1), 9-14.
· Haralabidis, S., E. Karagouni, S. Frydas, and E. Dotsika (1995). Immunoglobulin and cytokine profile in murine secondary hydatidosis. Parasite Immunol., 17, 625-530.
· Frydas, S., E. Karagouni, E. Dotsika, M. Reale, R.C. Barbacane, I. Vlemmas, G. Anogianakis, A. Trakatellis, and P. Conti (1996). Generation of TNF, IFN-, IL-6, IL-4 and IL-10 in mouse serum from trichinellosis: effect of the anti-inflammatory compound 4-deoxypyridoxine (4-DPD). Immunol. Let., 49, 179-184.
· Sideris, V., E. Karagouni, G. Papadopoulou, A. Garyfallou and E. Dotsika (1996). Canine visceral leishmaniasis in the great Athens area, Greece. Parasite, 3, 125-130.
· Chryssikopoulos, A., Th. Μantzavinos, N. Kanakas, E. Karagouni, E. Dotsika, and P.A. Zourlas (1996). Correlation of serum and follicular fluid concentrations of placenta protein 14 and CA-125 in in vitro fertilization-embryo transfer patients. Fertil. Steril., 66(4), 599-603.
· Dotsika, E., E. Karagouni, B. Sundquist, B. Morein, A. Morgan, and M. Villacres-Eriksson (1997). Ιnfluence of Quillaja saponaria triterpenoid content on the immunomodulatory capacity of Epstein-Barr virus (EBV) iscoms. Scand. J. Immunol. 45, 261-268.
· Papadopoulou, G., E. Karagouni and E. Dotsika (1998). ISCOMs vaccine against experimental Leishmaniasis. Vaccine, 16, 885-892.
· Karagouni, E., A. Chryssikopoulos, T. Mantzavinos, N. Kanakas and E. Dotsika (1998). Ιnterleukin-1 and interleukin-1 may affect the implantation rate of patients undergoing in vitro fertilization-embryo transfer. Fertil. Steril. 70, 553-559.
· Doukas, V., F. Athanasopoulou, E. Karagouni and E. Dotsika (1998). Aeromonas hydrophila infection in cultured see bass, Dicentrarchus labrax L. and Puntazzo puntazzo Cuvier from the Aegean sea. J. Fish Dis. 21, 317-320.
· Eliades, P., E. Karagouni, I. Stergiatou and K. Miras (1998). A simple method for the serodiagnosis of human hydatid disease based on a protein A/colloidal dye conjugate. J. Immunol. Meth. 218, 123-132.
· Frydas, S., Reale, M., Vacalis, D., Barbacane, R.C., Placido, F.C., Cataldo, I., Di Gioacchino, M., Karagouni, E., Dotsika, E., Anogiannakis, G., Trakatellis, A., Conti, P. (1999). IgG, IgG1 and IgM response in Trichinella spiralis-infected mice treated with 4-deoxypirydoxine or fed a Vitamin B6-deficient diet. Mol. Cell. Biochem. 194 (1-2), 47-52.
· Sideris, V., G. Papadopoulou, E. Dotsika and E. Karagouni (1999). Asymtomatic canine leishmaniasis in greater Athens area, Greece. Eur. J. Epidemiol. 15, 271-276.
· Tsagozis, P., E. Karagouni and E. Dotsika (2003). CD8+ T cells with parasite-specific cytotoxic activity and a Tc1profile of cytokine and chemokine secretion develop in experimental visceral leishmaniasis. Parasite Immunol. 25, 569-579.
· Frydas, S., M. Hatzistilianou, E. Karagouni, B. Madhappan, N. D’Orazio, G. Riccioni, F. Conti, G. Carratelli and D. Kempuraj (2003). Chemokines and parasites. Int. J. Immunopathol. Pharmacol. 16(3), 221-224.
· Papamattheou, M.G., J.G. Routsias, E.E. Karagouni, C. Sakarellos, M. Sakarellos-Daitsiotis, H.M. Moutsopoulos, A.G. Tzioufas and E.N. Dotsika (2004). T cell help is required to induce idiotypic-anti-idiotypic autoantibody network after immunization with complementary epitope 289-308aa of La/SSB autoantigen in non-autoimmune mice. Clinical & Experimental Immunology 135, 416-426.
· Tsagozis, P., Karagouni, E., Dotsika, E. (2004). Dendritic cells pulsed with peptides of gp63 induce differential protection against experimental cutaneous leishmaniasis. Int. J. Immunopathol. and Pharmacol. 17(3), 343-352.
· Athanassopoulou, F., E. Karagouni, E. Dotsika, V. Ragias, J. Tavla and P. Christofilloyanis (2004). Efficacy and toxicity of orally administrated anti-coccidial drugs for innovative treatments of Polysporoplasma sparis Sitja-Bobadilla & Alvarez-Pellitero 1885 infection in Sparus aurata L. Journal of Applied Ichthyology 20, 345-354.
· Athanassopoulou, F., E. Karagouni, E. Dotsika, V. Ragias, J. Tavla, P. Christofilloyanis and I. Vatsos (2004). Efficacy and toxicity of orally administrated anti-coccidial drugs for innovative treatments of Myxobolus sp. infection in Puntazzo puntazzo C. Diseases of Aquatic Organisms 62, 217-226.
· Frydas, S., E. Karagouni, M. Hatzistilianou, D. Kempuraj, S. Comani, C., Petrarca, T. Iezzi, N. Verna, P. Conti and M.L. Castellani (2004). Cytokines and allergic disorders: Revisited study. Int. J. Immunopathol. Pharmacol. 17(3), 233-235.
· Frydas, S., E. Karagouni, T. Iezzi, D. Kempuraj, C.M.V. Conti, W. Counter, N.G. Papadopoulou, J. Donelan, B. Madhappan, R. Letourneau, G. Carratelli, A. Grilli, F. Conti, G. Riccioni, D. Tripodi, F. Meneghini, I. Stellin and G. Sabatino (2004). Human genome project and parasitic infections. Eur. J. Inflamm. 2(1), 1-3.
· Frydas, S., E. Karagouni, E. Papadopoulos, M. Hatzistilianou, S. Pappas and N. Papaioannou (2004). Chemokines and their role in parasitic diseases. Eur. J. Inflamm. 2(2), 53-56.
· Tsagozis, P., V. Tseveleki, L. Probert, E. Dotsika and E. Karagouni (2004). Vaccination with plasmids encoding the Leishmania major gp63 glycoprotein and CD40L results in a partial suppression of the inflammatory reaction after experimental infection. Eur. J. Inflamm. 2(2), 91-96.
· Frydas, S., N. Papaioannou, M. Papazahariadou, M. Hatzistilianou, E. Karagouni, M. Trakatelli, G. Brellou, C. Petrarca, M.L. Castellani, P. Conti, G. Riccioni, A. Patruno and A. Grilli (2005). Inhibition of MCP-1 and MIP-2 chemokines in murine trichinellosis: effect of the anti-inflammatory compound L-mimosine. Int. J. Immunopathol. Pharmacol. 18(1), 121-132.
· Di Giannantonio, M., S. Frydas, D. Kempuraj, E. Karagouni, M. Hatzistilianou, C.M.V. Conti, W. Boucher, N. Papadopoulou, J., Donelan, J. Cao, B. Madhappan, P. Boscolo, C. Petrarca, L. Castellani, R. Quartesan, R. Doyle and F.M. Ferro (2005). Cytokines in stress. Int. J. Immunopathol. Pharmacol. 18(1), 1-5.
· Karagouni E., F. Athanassopoulou, P. Tsagozis, E. Ralli, T. Moustakareas, K. Lytra and E. Dotsika (2005). The impact of a successful anti-myxosporean treatment on the phagocyte functions of the juvenile and adult Sparus Aurata L. Int. J. Immunopathol. Pharmacol. 18(1), 121-132.
· Tsagozis, P., E. Karagouni and E. Dotsika (2005). Function of CD8+ T lymphocytes in a self-curing mouse model of visceral leishmaniasis. Parastiol. Int. 54(2), 139-146.
· Karagouni, E., F. Athanassopouloou, A. Lytra, C. Comis and E. Dotsika (2005). Antiparasitic and immunomodulatory effect of innovative treatments against Myxobolus sp. infection in Diplodus puntazzo. Vet. Parasitol. 134(3-4), 215-228.
· Golomazou, E., F. Athanassopoulou, E. Karagouni, S. Vagianou, H. Tsantilas and D. Karamanis (2006). Efficacy and toxixity of orally administrated anti-coccidial drug treatment on Enteromyxum leei infections in sharpsnout seabream (Diplodus puntazzo C.). Israeil J. Aquac. Bamidgeh 58(3), 157-169.
· Golomazou, E., F. Athanassopoulou, E. Karagouni, P. Tsagozis, H. Tsantilas and S. Vagianou (2006). Experimental transmission of Enteromyxun leei Diamant, Lom and Dykova, 1994 in sharpsnout sea bream, Diplodus puntazzo C. and the effect on some innate immune parameters. Aquaculture 260(1-4), 44-53.
· Golomazou, E., Athanssopoulou, F., Karagouni, E. and L. Kokkokiris (2009). The effect of seasonality on the health and growth of a newly recorded Myxobolus species infecting cultured Sharp Snout Seabream (Diplodus puntazzo C.). Turkish Journal of Veterinary and Animal Sciences 33(1), 1-5.
· Daoudaki, M., A. Diakou, S. Frydas, I. Fouzas, E. Karagouni, N. Vavatsi, and S. Haralabidis (2009). Vaccination with Trichinella spiralis antigens increase CD8+ peripheral T cells and enhance the TH2 immun response in Leishmania infantum challenged mice. International Journal of Immunopathology and Pharmacology 22(1), 169-174.
· Tsatchev, I., I.D. Kyriazis, S. Boutsini, E.E. Karagouni and E.N. Dotsika (2010). First report of canine visceral leishmaniasis in Bulgaria. Turkish Journal of Veterinary and Animal Sciences 34(5), 465-469.
· Symeonidou, I., Pappa, S., Kourelis, A., Anogeianaki, A., Frydas, I., Karagouni, E., Hatzistilianou, M. (2010). Microarray analysis of NF-kB signalling pathways in PBMC of mice infected by Trichinella spiralis. International Journal of Immunopathology and Pharmacology 23(3), 821-831.
· Symeonidou, I., Kourelis, A. Frydas, I., Karagouni, E., Anogeianaki, A., Hatzistilianou, M., Frydas, S. (2010). Modulation of NF-kB signalling pathways by parasites. Journal of Biological Regulators and Homeostatic Agents 34(5), 465-469.
· Symeonidou, I., Hatzistilianou, M., Papadopoulos, E., Dovas, C.I., Karagouni, E., Pappa, S., Pantzartzi, C.N., Kourelis, A., Frydas, S. (2011). Susceptibility and resistance to canine leishmaniose is associated to polymorphisms of the canine TNF-α gene. European Journal of Inflammation 9(1), 23-29.
· Agallou, M., Margaroni, M., Karagouni, E. (2011). Cellular vaccination with bone marrow-derived dendritic cells pulsed with a peptide of Leishmania infantum KMP-11 and CpG oligonucleotides induces protection in a murine model of visceral leishmaniasis. Vaccine 29(31), 5053-5064.
· Giannenas, I, Triantafillou, E., Stavrakakis, S., Margaroni, M., Mavridis, S., Steiner, T., Karagouni, E. (2012). Assessment of dietary supplementation with carvacrol or thymol containing feed additives on performance, intestinal microbiota and antioxidant status of rainbow trout (Oncorhynchus mykiss). Aquaculture, 305-353: 26-32.
· Agallou, M., D. Smirlis, K. Soteriadou and E. Karagouni (2012). Vaccination with Leishmania histone H1-pulsed dendritic cells confers protection in murine visceral leishmaniasis. Vaccine 30 (34), 5086-5093.
1 Protocol published
Detection of Antigen-specific T cells in Spleens of Vaccinated Mice Applying 3[H]-Thymidine Incorporation Assay and Luminex Multiple Cytokine Analysis Technology
Authors:  Maria Agallou and Evdokia Karagouni, date: 06/05/2019, view: 278, Q&A: 0
For many infectious diseases T cells are an important part of naturally acquired protective immune responses, and inducing these by vaccination has been the aim of much research. Here, we describe a protocol for the analysis of vaccine-induced ...
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