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Qingyu Wu
Cleveland Clinic, USA
2 protocols
Belinda Willard
1 protocol
Meiling He
1 protocol
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Hao Wang
Fudan University, China
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2
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2 Protocols published
Cross-linking, Immunoprecipitation and Proteomic Analysis to Identify Interacting Proteins in Cultured Cells
Authors:
Hao Wang
,
Meiling He
,
Belinda Willard
and
Qingyu Wu
,
date:
06/05/2019,
view:
353,
Q&A:
0
Extracellular expression is essential for the function of secreted and cell surface proteins. Proper intracellular trafficking depends on protein interactions in multiple subcellular compartments. Co-immunoprecipitation and the yeast two-hybrid ...
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Glucosidase Inhibition to Study Calnexin-assisted Glycoprotein Folding in Cells
Authors:
Hao Wang
and
Qingyu Wu
,
date:
06/05/2019,
view:
246,
Q&A:
0
Calnexin is a chaperone protein that plays a critical role in glycoprotein folding in the endoplasmic reticulum (ER). The function of calnexin depends on its binding to monoglucosylated oligosaccharides on nascent glycoproteins, whereas the ...
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