Boris Maček 1 protocol

Joyita Mukherjee Department of Neuromuscular Diseases, UCL, 2019-
1 protocol

Ralf-Peter Jansen
  • Professor and Chair of Biochemistry, Dept. of Pharmaceutical Sciences and Biochemistry, University of Tuebingen
Research focus
  • -
  • 1 Author merit


Dr. rer.nat., University of Heidelberg, Germany, 1993


Since 2010
• Béthune, J., Jansen, R. P., Feldbrugge, M. and Zarnack, K. (2019). Membrane-Associated RNA-Binding Proteins Orchestrate Organelle-Coupled Translation. Trends Cell Biol 29(2): 178-188.
• Manchalu, S., Mittal, N., Spang, A. and Jansen, R. P. (2019). Local translation of yeast ERG4 mRNA at the endoplasmic reticulum requires the brefeldin A resistance protein Bfr1. RNA 25(12): 1661-1672.
• Mukherjee, J., Hermesh, O., Eliscovich, C., Nalpas, N., Franz-Wachtel, M., Macek, B. and Jansen, R. P. (2019). β-Actin mRNA interactome mapping by proximity biotinylation. Proc Natl Acad Sci U S A 116(26): 12863-12872.
• Singer-Krüger, B., Frohlich, T., Franz-Wachtel, M., Nalpas, N., Macek, B. and Jansen, R. P. (2019). APEX2-mediated proximity labeling resolves protein networks in Saccharomyces cerevisiae cells. FEBS J.
• Cheng, M. H. K., Hoffmann, P. C., Franz-Wachtel, M., Sparn, C., Seng, C., Macek, B. and Jansen, R. P. (2018). The RNA-Binding Protein Scp160p Facilitates Aggregation of Many Endogenous Q/N-Rich Proteins. Cell Rep 24(1): 20-26.
• Niessing, D., Jansen, R. P., Pohlmann, T. and Feldbrügge, M. (2018). mRNA transport in fungal top models. Wiley Interdiscip Rev RNA 9(1).
• Syed, M. I., Moorthy, B. T., Jenner, A., Fetka, I. and Jansen, R. P. (2018). Signal sequence-independent targeting of MID2 mRNA to the endoplasmic reticulum by the yeast RNA-binding protein Khd1p. FEBS Lett 592(11): 1870-1881.
• Cheng, M. H. and Jansen, R. P. (2017). A jack of all trades: the RNA-binding protein vigilin. Wiley Interdiscip Rev RNA 8(6).
• Edelmann, F. T., Schlundt, A., Heym, R. G., Jenner, A., Niedner-Boblenz, A., Syed, M. I., Paillart, J. C., Stehle, R., Janowski, R., Sattler, M., Jansen, R. P. and Niessing, D. (2017). Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex. Nat Struct Mol Biol 24(2): 152-161.
• Jansen, R. P., Niessing, D., Baumann, S. and Feldbrügge, M. (2014). mRNA transport meets membrane traffic. Trends Genet 30(9): 408-417.
• Hermesh, O., Genz, C., Yofe, I., Sinzel, M., Rapaport, D., Schuldiner, M. and Jansen, R. P. (2014). Yeast phospholipid biosynthesis is linked to mRNA localization. J Cell Sci 127(Pt 15): 3373-3381.
• Hirschmann, W. D., Westendorf, H., Mayer, A., Cannarozzi, G., Cramer, P. and Jansen, R. P. (2014). Scp160p is required for translational efficiency of codon-optimized mRNAs in yeast. Nucleic Acids Res 42(6): 4043-4055.
• Pfeiffer-Guglielmi, B., Dombert, B., Jablonka, S., Hausherr, V., van Thriel, C., Schöbel, N. and Jansen, R. P. (2014). Axonal and dendritic localization of mRNAs for glycogen-metabolizing enzymes in cultured rodent neurons. BMC Neurosci 15: 70.
• Genz, C., Fundakowski, J., Hermesh, O., Schmid, M. and Jansen, R. P. (2013). Association of the yeast RNA-binding protein She2p with the tubular endoplasmic reticulum depends on membrane curvature. J Biol Chem 288(45): 32384-32393.
• Hermesh, O. and Jansen, R. P. (2013). Take the (RN)A-train: localization of mRNA to the endoplasmic reticulum. Biochim Biophys Acta 1833(11): 2519-2525.
• Niedner, A., Muller, M., Moorthy, B. T., Jansen, R. P. and Niessing, D. (2013). Role of Loc1p in assembly and reorganization of nuclear ASH1 messenger ribonucleoprotein particles in yeast. Proc Natl Acad Sci U S A 110(52): E5049-5058.
• Fundakowski, J., Hermesh, O. and Jansen, R. P. (2012). Localization of a subset of yeast mRNAs depends on inheritance of endoplasmic reticulum. Traffic 13(12): 1642-1652.
• Jansen, R. P. and Niessing, D. (2012). Assembly of mRNA-protein complexes for directional mRNA transport in eukaryotes--an overview. Curr Protein Pept Sci 13(4): 284-293.
• Müller, M., Heym, R. G., Mayer, A., Kramer, K., Schmid, M., Cramer, P., Urlaub, H., Jansen, R. P. and Niessing, D. (2011). A cytoplasmic complex mediates specific mRNA recognition and localization in yeast. PLoS Biol 9(4): e1000611.
• Heuck, A., Fetka, I., Brewer, D. N., Huls, D., Munson, M., Jansen, R. P. and Niessing, D. (2010). The structure of the Myo4p globular tail and its function in ASH1 mRNA localization. J Cell Biol 189(3): 497-510.
1 Protocol published
利用 RNA-BioID鉴定小鼠胚胎成纤维细胞中RNA相互作用体
Authors:  Joyita Mukherjee, Mirita Franz-Wachtel, Boris Maček and Ralf-Peter Jansen, date: 01/05/2020, view: 3034, Q&A: 0
Cytoplasmic localization of mRNAs is common to all organisms and serves the spatial expression of genes. Cis-acting RNA signals (mostly found in the mRNA’s 3'-UTR), called zipcodes recruit trans-acting RNA-binding proteins that facilitate the ...
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