Kazuo Kurokawa
  • Senior Research Scientist, Live Cell Super-Resolution Imaging Research team, Riken Center for Advanced Photonics
Research focus
  • Cell biology
  • Membrane traffic
  • 1 Author merit

Education

Ph. D in Bioscience, Nara institute of science and technology, Japan, 2000

Lab information

Live Cell Super-Resolution Imaging Research Team
http://sclim.riken.jp/en/

Publications

Since 2010
• Fujii, S., Kurokawa, K., Inaba, R., Hiramatsu, N., Tago, T., Nakamura, Y., Nakano, A., Satoh, T. and Satoh, A. K. (2020). Recycling endosomes attach to the trans-side of Golgi stacks in Drosophila and mammalian cells. J Cell Sci 133(4).
• Matsuura, R., Inabe, K., Otsuki, H., Kurokawa, K., Dohmae, N. and Aida, Y. (2019). Three YXXL Sequences of a Bovine Leukemia Virus Transmembrane Protein are Independently Required for Fusion Activity by Controlling Expression on the Cell Membrane. Viruses 11(12).
• Ishii, A., Kurokawa, K., Hotta, M., Yoshizaki, S., Kurita, M., Koyama, A., Nakano, A. and Kimura, Y. (2019). Role of Atg8 in the regulation of vacuolar membrane invagination. Sci Rep 9(1): 14828.
• Tojima, T., Suda, Y., Ishii, M., Kurokawa, K. and Nakano, A. (2019). Spatiotemporal dissection of the trans-Golgi network in budding yeast. J Cell Sci 132(15).
• Maeda, M., Kurokawa, K., Katada, T., Nakano, A. and Saito, K. (2019). COPII proteins exhibit distinct subdomains within each ER exit site for executing their functions. Sci Rep 9(1): 7346.
• Kurokawa, K., Osakada, H., Kojidani, T., Waga, M., Suda, Y., Asakawa, H., Haraguchi, T. and Nakano, A. (2019). Visualization of secretory cargo transport within the Golgi apparatus. J Cell Biol 218(5): 1602-1618.
• Kurokawa, K. and Nakano, A. (2019). The ER exit sites are specialized ER zones for the transport of cargo proteins from the ER to the Golgi apparatus. J Biochem 165(2): 109-114.
• Suda, Y., Tachikawa, H., Inoue, I., Kurita, T., Saito, C., Kurokawa, K., Nakano, A. and Irie, K. (2018). Activation of Rab GTPase Sec4 by its GEF Sec2 is required for prospore membrane formation during sporulation in yeast Saccharomyces cerevisiae. FEMS Yeast Res 18(1).
• Suda, Y., Kurokawa, K. and Nakano, A. (2017). Regulation of ER-Golgi Transport Dynamics by GTPases in Budding Yeast. Front Cell Dev Biol 5: 122.
• Kobayashi, K., Suemasa, F., Sagara, H., Nakamura, S., Ino, Y., Kobayashi, K., Hiramatsu, H., Haraguchi, T., Kurokawa, K., Todo, T., Nakano, A. and Iba, H. (2017). MiR-199a Inhibits Secondary Envelopment of Herpes Simplex Virus-1 Through the Downregulation of Cdc42-specific GTPase Activating Protein Localized in Golgi Apparatus. Sci Rep 7(1): 6650.
• Ishii, M., Suda, Y., Kurokawa, K. and Nakano, A. (2016). COPI is essential for Golgi cisternal maturation and dynamics. J Cell Sci 129(17): 3251-3261.
• Kurokawa, K., Suda, Y. and Nakano, A. (2016). Sar1 localizes at the rims of COPII-coated membranes in vivo. J Cell Sci 129(17): 3231-3237.
• Iwai, M., Yokono, M., Kurokawa, K., Ichihara, A. and Nakano, A. (2016). Live-cell visualization of excitation energy dynamics in chloroplast thylakoid structures. Sci Rep 6: 29940.
• Kurokawa, K., Okamoto, M. and Nakano, A. (2014). Contact of cis-Golgi with ER exit sites executes cargo capture and delivery from the ER. Nat Commun 5: 3653.
• Kurokawa, K., Ishii, M., Suda, Y., Ichihara, A. and Nakano, A. (2013). Live cell visualization of Golgi membrane dynamics by super-resolution confocal live imaging microscopy. Methods Cell Biol 118: 235-242.
• Suda, Y., Kurokawa, K., Hirata, R. and Nakano, A. (2013). Rab GAP cascade regulates dynamics of Ypt6 in the Golgi traffic. Proc Natl Acad Sci U S A 110(47): 18976-18981.
• Okamoto, M., Kurokawa, K., Matsuura-Tokita, K., Saito, C., Hirata, R. and Nakano, A. (2012). High-curvature domains of the ER are important for the organization of ER exit sites in Saccharomyces cerevisiae. J Cell Sci 125(Pt 14): 3412-3420.
• Maeda, Y., Yumoto, M., Saito, N., Ogawa, T., Kurokawa, K., Nakano, A., Yamashita, M. and Wada, S. (2010). Broadly tunable UV-blue picosecond pulsed laser and its application for biological imaging. Optical Review 17(3): 305-308.
• Yamashita, M., Kurokawa, K., Sato, Y., Yamagata, A., Mimura, H., Yoshikawa, A., Sato, K., Nakano, A. and Fukai, S. (2010). Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3. Nat Struct Mol Biol 17(2): 180-186.
1 Protocol published
Live-cell Imaging by Super-resolution Confocal Live Imaging Microscopy (SCLIM): Simultaneous Three-color and Four-dimensional Live Cell Imaging with High Space and Time Resolution
Authors:  Kazuo Kurokawa and Akihiko Nakano, date: 09/05/2020, view: 695, Q&A: 0
Many questions in cell biology can be solved by state-of-the-art technology of live cell imaging. One good example is the mechanism of membrane traffic, in which small membrane carriers are rapidly moving around in the cytoplasm to deliver cargo ...
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