Nicholas Pugh School of Life Sciences, Anglia Ruskin University, Cambridge
1 protocol

Laura Burzynski Department of Medicine, University of Cambridge,2017-0
1 protocol

Murray C.H. Clarke
  • BHF Senior Fellow, Dept. Medicine, University of Cambridge, 2003-2019
Research focus
  • -
  • 1 Author merit


PhD, University of Edinburgh, Scotland, 2002


• Burzynski, L. C. and Clarke, M. C. H. (2019). Death Is Coming and the Clot Thickens, as Pyroptosis Feeds the Fire. Immunity 50(6): 1339-1341.
• Burzynski L. C., Humphry, M., Pyrillou, K., Wiggins, K. A., Chan, J. N. E, Figg, N. Kitt, L. L., Summers, C., Tatham, K. C., Martin, P. B., Bennett, M. R. and Clarke, M. C. H. (2019). The coagulation and immune system are directly linked through the activation of interleukin-1α by thrombin. Immunity 50, 1033–1042.
• Wiggins, K. A., Parry, A. J., Cassidy, L. D., Humphry, M., Webster, S. J., Goodall, J. C., Narita, M. and Clarke, M. C. H. (2019). IL-1alpha cleavage by inflammatory caspases of the noncanonical inflammasome controls the senescence-associated secretory phenotype. Aging Cell 18(3): e12946.
• Basatemur, G. L., Jorgensen, H. F., Clarke, M. C. H., Bennett, M. R. and Mallat, Z. (2019). Vascular smooth muscle cells in atherosclerosis. Nat Rev Cardiol.
• Wiggins, K. A. and Clarke, M. C. (2019). Senescence utilises inflammatory caspases to drive the SASP. Aging (Albany NY) 11(12): 3891-3892.
• Burzynski L. C., Pugh, N. and Clarke, M. C. H. (2019). Platelet isolation and activation assays. Bio-protocol, In Press.
• Cassidy, L., Young, A. R. J, Young, C. N. J., Soilleux, E. J., Fielder, E., Weigand, B. M., Brais, R., Wiggins, K. A., Clarke, M. C. H., Jurk, D., Passos, J. F. and Narita, M. (2019).Temporal inhibition of autophagy reveals segmental reversal of aging with increased cancer risk.
• Bennett, M. R. and Clarke, M. C. (2017). Killing the old: cell senescence in atherosclerosis. Nat Rev Cardiol 14(3): 132.
• Wang, J., Uryga, A. K., Reinhold, J., Figg, N., Baker, L., Finigan, A., Gray, K., Kumar, S., Clarke, M. and Bennett, M. (2015). Vascular Smooth Muscle Cell Senescence Promotes Atherosclerosis and Features of Plaque Vulnerability. Circulation 132(20): 1909-1919.
• Burzynski, L. C., Humphry, M., Bennett, M. R. and Clarke, M. C. (2015). Interleukin-1alpha Activity in Necrotic Endothelial Cells Is Controlled by Caspase-1 Cleavage of Interleukin-1 Receptor-2: IMPLICATIONS FOR ALLOGRAFT REJECTION. J Biol Chem 290(41): 25188-25196.
• Gardner, S. E., Humphry, M., Bennett, M. R. and Clarke, M. C. (2015). Senescent Vascular Smooth Muscle Cells Drive Inflammation Through an Interleukin-1alpha-Dependent Senescence-Associated Secretory Phenotype. Arterioscler Thromb Vasc Biol 35(9): 1963-1974.
• Maguire, J. J., Jones, K. L., Kuc, R. E., Clarke, M. C., Bennett, M. R. and Davenport, A. P. (2014). The CCR5 chemokine receptor mediates vasoconstriction and stimulates intimal hyperplasia in human vessels in vitro. Cardiovasc Res 101(3): 513-521.
• Yu, E., Calvert, P. A., Mercer, J. R., Harrison, J., Baker, L., Figg, N. L., Kumar, S., Wang, J. J., Hurst, L. A., Obaid, D. R., Logan, A., West, N. E., Clarke, M. C., Vidal-Puig, A., Murphy, M. P. and Bennett, M. R. (2014). Response to letter regarding article, "Mitochondrial DNA damage can promote atherosclerosis independently of reactive oxygen species through effects on smooth muscle cells and monocytes and correlates with higher-risk plaques in humans". Circulation 129(12): e408.
• Zheng, Y., Humphry, M., Maguire, J. J., Bennett, M. R. and Clarke, M. C. (2013). Intracellular interleukin-1 receptor 2 binding prevents cleavage and activity of interleukin-1alpha, controlling necrosis-induced sterile inflammation. Immunity 38(2): 285-295.
• Bennett, M., Yu, H. and Clarke, M. (2012). Signalling from dead cells drives inflammation and vessel remodelling. Vascul Pharmacol 56(5-6): 187-192.
• Zheng, Y., Gardner, S. E. and Clarke, M. C. (2011). Cell death, damage-associated molecular patterns, and sterile inflammation in cardiovascular disease. Arterioscler Thromb Vasc Biol 31(12): 2781-2786.
• Yu, H., Clarke, M. C., Figg, N., Littlewood, T. D. and Bennett, M. R. (2011). Smooth muscle cell apoptosis promotes vessel remodeling and repair via activation of cell migration, proliferation, and collagen synthesis. Arterioscler Thromb Vasc Biol 31(11): 2402-2409.
• Calvert, P. A., Liew, T. V., Gorenne, I., Clarke, M., Costopoulos, C., Obaid, D. R., O'Sullivan, M., Shapiro, L. M., McNab, D. C., Densem, C. G., Schofield, P. M., Braganza, D., Clarke, S. C., Ray, K. K., West, N. E. and Bennett, M. R. (2011). Leukocyte telomere length is associated with high-risk plaques on virtual histology intravascular ultrasound and increased proinflammatory activity. Arterioscler Thromb Vasc Biol 31(9): 2157-2164.
• Yu, H., Stoneman, V., Clarke, M., Figg, N., Xin, H. B., Kotlikoff, M., Littlewood, T. and Bennett, M. (2011). Bone marrow-derived smooth muscle-like cells are infrequent in advanced primary atherosclerotic plaques but promote atherosclerosis. Arterioscler Thromb Vasc Biol 31(6): 1291-1299.
• Clarke, M. C., Talib, S., Figg, N. L. and Bennett, M. R. (2010). Vascular smooth muscle cell apoptosis induces interleukin-1-directed inflammation: effects of hyperlipidemia-mediated inhibition of phagocytosis. Circ Res 106(2): 363-372.
• Clarke, M. C., and Bennett, M. R. (2009). Cause or Consequence: What Does Macrophage Apoptosis Do in Atherosclerosis? ATVB. 29:153.
• Clarke, M. C., Littlewood, T. D., Figg, N., Maguire, J. J., Davenport, A. P., Goddard, M. and Bennett, M. R. (2008). Chronic apoptosis of vascular smooth muscle cells accelerates atherosclerosis and promotes calcification and medial degeneration. Circ Res 102(12): 1529-1538.
• Clarke, M., Bennett, M. and Littlewood, T. (2007). Cell death in the cardiovascular system. Heart 93(6): 659-664.
• Clarke, M. C., Figg, N., Maguire, J. J., Davenport, A. P., Goddard, M., Littlewood, T. D. and Bennett, M. R. (2006). Apoptosis of vascular smooth muscle cells induces features of plaque vulnerability in atherosclerosis. Nat Med 12(9): 1075-1080.
• Clarke, M. and Bennett, M. (2006). Defining the role of vascular smooth muscle cell apoptosis in atherosclerosis. Cell Cycle 5(20): 2329-2331.
• Clarke, M. and Bennett, M. (2006). The emerging role of vascular smooth muscle cell apoptosis in atherosclerosis and plaque stability. Am J Nephrol 26(6): 531-535.
• Clarke, M. and Bennett, M. (2006). The emerging role of vascular smooth muscle cell apoptosis in atherosclerosis and plaque stability. Am J Nephrol 26(6): 531-535.
• Wickenden, J. A., Clarke, M. C., Rossi, A. G., Rahman, I., Faux, S. P., Donaldson, K. and MacNee, W. (2003). Cigarette smoke prevents apoptosis through inhibition of caspase activation and induces necrosis. Am J Respir Cell Mol Biol 29(5): 562-570.
• Clarke, M. C., Savill, J., Jones, D. B., Noble, B. S. and Brown, S. B. (2003). Compartmentalized megakaryocyte death generates functional platelets committed to caspase-independent death. J Cell Biol 160(4): 577-587.
• Brown, S. B., Clarke, M. C., Magowan, L., Sanderson, H. and Savill, J. (2000). Constitutive death of platelets leading to scavenger receptor-mediated phagocytosis. A caspase-independent cell clearance program. J Biol Chem 275(8): 5987-5996.
1 Protocol published
Platelet Isolation and Activation Assays
Authors:  Laura C. Burzynski, Nicholas Pugh and Murray C.H. Clarke, date: 10/20/2019, view: 484, Q&A: 0
Platelets regulate hemostasis and are the key determinants of pathogenic thrombosis following atherosclerotic plaque rupture. Platelets circulate in an inactive state, but become activated in response to damage to the endothelium, which exposes ...
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