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Mingyi Xie
Biochemistry and Molecular Biology, University of Florida, USA, USA,
2 protocols
Tianqi Wei
1 protocol
Peike Sheng
1 protocol
Christopher Fields
Department of Biochemistry and Molecular Biology, University of Florida, USA, USA,
1 protocol
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Bret Miller
Research focus
Molecular biology
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1 Protocol published
Northern Blot with IR Fluorescent Probes: Strategies for Probe Preparation
Authors:
Christopher Fields
,
Peike Sheng
,
Bret Miller
,
Tianqi Wei
and
Mingyi Xie
,
date:
04/20/2019,
view:
6152,
Q&A:
1
Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern). Our method is as ...
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Lonneke Verboon
Lonneke Verboon
It is maybe clear when you have done Northern blot many times. But which marker/ladder do I use on the blot? dsRNA or ssRNA or both?
8/1/2019 12:59:52 AM
Reply
Mingyi Xie
Biochemistry and Molecular Biology, University of Florida, USA, USA,
We typically use NEB microRNA ladder (N2102S) and low range ssRNA ladder (N0364S) for microRNA northern.
2019-08-02 05:55:16
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