Caroline Grönwall Department of Medicine, Rheumatology Division, Karolinska University Hospital. Center for Molecular Medicine, Stockholm
1 protocol

Vivianne Malmström Department of Medicine, Rheumatology Division, Karolinska University Hospital. Center for Molecular Medicine, Stockholm
1 protocol

Johanna Steen Department of Medicine, Rheumatology Division, Karolinska University Hospital. Center for Molecular Medicine, Stockholm
1 protocol

Peter Sahlström Department of Medicine, Rheumatology Division, Karolinska University Hospital. Center for Molecular Medicine, Stockholm
1 protocol

khaled amara
  • Research scientist, Karolinska Institute
Research focus
  • Immunology
  • 1 Author merit

Education

in Biological Sciences and Biotechnology, Higher Institute of Biotechnology of Monastir, Tunisia, 2009

Publications

• Ramsköld D, Parodis I, Lakshmikanth T, Sippl N, Khademi M, Chen Y, Zickert A, Mikeš J, Achour A, Amara K, Piehl F, Brodin P, Gunnarsson I, Malmström V. B cell alterations during BAFF inhibition with belimumab in SLE. EBioMedicine. 2019 Feb;40:517-527. doi: 10.1016/j.ebiom.2018.12.035.
• Epub 2018 Dec 26. PubMed PMID: 30593436; PubMed Central PMCID: PMC6412067. 2: Grosso G, Sippl N, Kjellström B, Amara K, de Faire U, Elvin K, Lindahl B, Näsman P, Rydén L, Norhammar A, Svenungsson E. Antiphospholipid Antibodies in Patients With Myocardial Infarction. Ann Intern Med. 2018 Oct 23. doi: 10.7326/M18-2130. [Epub ahead of print] PubMed PMID: 30357274.
• Titcombe PJ, Wigerblad G, Sippl N, Zhang N, Shmagel AK, Sahlström P, Zhang Y, Barsness LO, Ghodke-Puranik Y, Baharpoor A, Hansson M, Israelsson L, Skriner K, Niewold TB, Klareskog L, Svensson CI, Amara K, Malmström V, Mueller DL. Pathogenic Citrulline-Multispecific B Cell Receptor Clades in Rheumatoid Arthritis. Arthritis Rheumatol. 2018 Dec;70(12):1933-1945. doi: 10.1002/art.40590. Epub 2018 Oct 18. PubMed PMID: 29927106; PubMed Central PMCID: PMC6261688.
• Lloyd KA, Steen J, Amara K, Titcombe PJ, Israelsson L, Lundström SL, Zhou D, Zubarev RA, Reed E, Piccoli L, Gabay C, Lanzavecchia A, Baeten D, Lundberg K, Mueller DL, Klareskog L, Malmström V, Grönwall C. Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: Implications for B-cell selection. Eur J Immunol. 2018 Jun;48(6):1030-1045. doi: 10.1002/eji.201747446. Epub 2018 Apr 6. PubMed PMID: 29512823.
• Amara K, Clay E, Yeo L, Ramsköld D, Spengler J, Sippl N, Cameron J, Israelsson L, Titcombe PJ, Grönwall C, Sahbudin I, Filer A, Raza K, Malmström V, Scheel-Toellner D. Immunoglobulin characteristics and RNAseq data of FcRL4+ B cells sorted from synovial fluid and tissue of patients with rheumatoid arthritis. Data Brief. 2017 Jun 7;13:356-370. doi: 10.1016/j.dib.2017.06.009. eCollection 2017 Aug. PubMed PMID: 28664171; PubMed Central PMCID: PMC5480816.
• Grönwall C, Amara K, Hardt U, Krishnamurthy A, Steen J, Engström M, Sun M, Ytterberg AJ, Zubarev RA, Scheel-Toellner D, Greenberg JD, Klareskog L, Catrina AI, Malmström V, Silverman GJ. Autoreactivity to malondialdehyde-modifications in rheumatoid arthritis is linked to disease activity and synovial pathogenesis. J Autoimmun. 2017 Nov;84:29-45. doi: 10.1016/j.jaut.2017.06.004. Epub 2017 Jun 21. PubMed PMID: 28647488.
• Amara K, Clay E, Yeo L, Ramsköld D, Spengler J, Sippl N, Cameron JA, Israelsson L, Titcombe PJ, Grönwall C, Sahbudin I, Filer A, Raza K, Malmström V, Scheel-Toellner D. B cells expressing the IgA receptor FcRL4 participate in the autoimmune response in patients with rheumatoid arthritis. J Autoimmun. 2017 Jul; 81:34-43. doi: 10.1016/j.jaut.2017.03.004. Epub 2017 Mar 24. PubMed PMID: 28343748; PubMed Central PMCID: PMC5473332.
• Wigerblad G, Bas DB, Fernades-Cerqueira C, Krishnamurthy A, Nandakumar KS, Rogoz K, Kato J, Sandor K, Su J, Jimenez-Andrade JM, Finn A, Bersellini Farinotti A, Amara K, Lundberg K, Holmdahl R, Jakobsson PJ, Malmström V, Catrina AI, Klareskog L, Svensson CI. Autoantibodies to citrullinated proteins induce joint pain independent of inflammation via a chemokine-dependent mechanism. Ann Rheum Dis. 2016 Apr;75(4):730-8. doi: 10.1136/annrheumdis-2015-208094. Epub 2015 Nov 27. Erratum in: Ann Rheum Dis. 2019 Jun;78(6):865. PubMed PMID: 26613766; PubMed Central PMCID: PMC4819624.
• Krishnamurthy A, Joshua V, Haj Hensvold A, Jin T, Sun M, Vivar N, Ytterberg AJ, Engström M, Fernandes-Cerqueira C, Amara K, Magnusson M, Wigerblad G, Kato J, Jiménez-Andrade JM, Tyson K, Rapecki S, Lundberg K, Catrina SB, Jakobsson PJ, Svensson C, Malmström V, Klareskog L, Wähämaa H, Catrina AI. Identification of a novel chemokine-dependent molecular mechanism underlying rheumatoid arthritis-associated autoantibody-mediated bone loss. Ann Rheum Dis. 2016 Apr;75(4):721-9. doi: 10.1136/annrheumdis-2015-208093. Epub 2015 Nov 26. Erratum in: Ann Rheum Dis. 2019 Jun;78(6):866. PubMed PMID: 26612338; PubMed Central PMCID: PMC4819614.
• Harre U, Lang SC, Pfeifle R, Rombouts Y, Frühbeißer S, Amara K, Bang H, Lux A, Koeleman CA, Baum W, Dietel K, Gröhn F, Malmström V, Klareskog L, Krönke G, Kocijan R, Nimmerjahn F, Toes RE, Herrmann M, Scherer HU, Schett G. Glycosylation of immunoglobulin G determines osteoclast differentiation and bone loss. Nat Commun. 2015 Mar 31;6:6651. doi: 10.1038/ncomms7651. PubMed PMID: 25825024; PubMed Central PMCID: PMC4389255.
• Ben Dhiab M, Ziadi S, Ksiaa F, Louhichi T, Ben Gacem R, Ben Zineb A, Amara K, Hachana M, Trimeche M. Methylation of miR124a-1, miR124a-2, and miR124a-3 in Hodgkin lymphoma. Tumour Biol. 2015 Mar;36(3):1963-71. doi: 10.1007/s13277-014-2802-3. Epub 2014 Nov 15. PubMed PMID: 25394899.
• Fiskesund R, Steen J, Amara K, Murray F, Szwajda A, Liu A, Douagi I, Malmström V, Frostegård J. Naturally occurring human phosphorylcholine antibodies are predominantly products of affinity-matured B cells in the adult. J Immunol. 2014 May 15;192(10):4551-9. doi: 10.4049/jimmunol.1303035. Epub 2014 Apr 11. PubMed PMID: 24729615.
• Klareskog L, Amara K, Malmström V. Adaptive immunity in rheumatoid arthritis: anticitrulline and other antibodies in the pathogenesis of rheumatoid arthritis. Curr Opin Rheumatol. 2014 Jan;26(1):72-9. doi: 10.1097/BOR.0000000000000016. Review. PubMed PMID: 24257366.
• Hachana M, Amara K, Ziadi S, Gacem RB, Korbi S, Trimeche M. Investigation of human JC and BK polyomaviruses in breast carcinomas. Breast Cancer Res Treat. 2012 Jun;133(3):969-77. doi: 10.1007/s10549-011-1876-5. Epub 2011 Nov 23. PubMed PMID: 22108781.
• Hachana M, Amara K, Ziadi S, Romdhane E, Gacem RB, Trimeche M. Investigation of Epstein-Barr virus in breast carcinomas in Tunisia. Pathol Res Pract. 2011 Nov 15;207(11):695-700. doi: 10.1016/j.prp.2011.09.007. Epub 2011 Oct 24. PubMed PMID: 22024152.
• Hachana M, Ziadi S, Amara K, Toumi I, Korbi S, Trimeche M. No evidence of human papillomavirus DNA in breast carcinoma in Tunisian patients. Breast. 2010 Dec;19(6):541-4. doi: 10.1016/j.breast.2010.05.007. Epub 2010 Jun 12. PubMed PMID: 20547456.
• Amara K, Ziadi S, Hachana M, Soltani N, Korbi S, Trimeche M. DNA methyltransferase DNMT3b protein overexpression as a prognostic factor in patients with diffuse large B-cell lymphomas. Cancer Sci. 2010 Jul;101(7):1722-30. doi: 10.1111/j.1349-7006.2010.01569.x. Epub 2010 Mar 19. PubMed PMID: 20398054.
• Amara K, Trimeche M, Ziadi S, Laatiri A, Hachana M, Korbi S. Prognostic significance of aberrant promoter hypermethylation of CpG islands in patients with diffuse large B-cell lymphomas. Ann Oncol. 2008 Oct;19(10):1774-86. doi: 10.1093/annonc/mdn374. Epub 2008 Jun 6. PubMed PMID: 18539616.
• Amara K, Trimeche M, Ziadi S, Laatiri A, Mestiri S, Sriha B, Mokni M, Korbi S. Presence of simian virus 40 in diffuse large B-cell lymphomas in Tunisia correlates with germinal center B-cell immunophenotype, t(14;18) translocation, and P53 accumulation. Mod Pathol. 2008 Mar;21(3):282-96. doi: 10.1038/modpathol.3800993. Epub 2007 Dec 28. PubMed PMID: 18165800.
• Amara K, Trimeche M, Ziadi S, Laatiri A, Hachana M, Sriha B, Mokni M, Korbi S. Presence of simian virus 40 DNA sequences in diffuse large B-cell lymphomas in Tunisia correlates with aberrant promoter hypermethylation of multiple tumor suppressor genes. Int J Cancer. 2007 Dec 15;121(12):2693-702. PubMed PMID: 17724719.
• Amara K, Trimeche M, Ziadi S, Sriha B, Mokni M, Korbi S. PCR-based clonality analysis of B-cell lymphomas in paraffin-embedded tissues: diagnostic value of immunoglobulin kappa and lambda light chain gene rearrangement investigation. Pathol Res Pract. 2006;202(6):425-31. Epub 2006 May 4. PubMed PMID: 16677778.
1 Protocol published
A Refined Protocol for Identifying Citrulline-specific Monoclonal Antibodies from Single Human B Cells from Rheumatoid Arthritis Patient Material
We describe here a detailed, refined protocol for the generation of citrulline-specific monoclonal antibodies from single human B cells from rheumatoid arthritis (RA) patients. This protocol provides a detailed guide of the procedure starting from ...
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