Allen R. Buskirk —BIO-PROTOCOL

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Fuad Mohammad Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland
1 protocol

Allen R. Buskirk

Research scientist, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland

Research focus

Microbiology

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Protocol for Ribosome Profiling in Bacteria

Authors: Fuad Mohammad and Allen R. Buskirk, date: 12/20/2019, view: 4141, Q&A: 1

Ribosome profiling provides information on the position of ribosomes on mRNA on a genomic scale. Although this information is often used to detect changes in gene expression under different conditions, it also has great potential for yielding ...

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Yonatan Chemla

MIT

Hi, one technical question regarding the primer NI-NI-9. if one orders PAGE Ultramer DNA oligo from IDT, you cannot specify that it will be 250nmol like the protocol says. Did you order a 250 nmol DNA oligo with PAGE purification or a PAGE Ultramer?

Thanks,

Yonatan

11/12/2020 11:00:44 AM Reply

Yonatan Chemla

MIT

Thank you, Allen. This protocol and studies behind it are important and useful, so thank you for those as well!

Best,

Yonatan

2020-11-24 05:24:43

Allen Buskirk

Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland

Yonatan,

250 nmol DNA Oligo, PAGE purified and 5'-phosphorylated. It was not ordered as an Ultramer.

Allen

2020-11-23 08:46:25