Zaneta Matuszek —BIO-PROTOCOL

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Tao Pan University of Chicago
1 protocol

Zaneta Matuszek

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1 Protocol published

Quantification of Queuosine Modification Levels in tRNA from Human Cells Using APB Gel and Northern Blot

Authors: Zaneta Matuszek and Tao Pan, date: 03/20/2019, view: 498, Q&A: 0

Queuosine (Q) is a hypermodified base in the wobble anticodon position of tRNAs coding for the amino acids Tyr, His, Asn, and Asp. tRNA Q-modification is introduced by a queuine tRNA-ribosyltransferase (TGT) that replaces the guanine base at G34 at ...

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