Tomoyuki Yamaguchi
  • Project Associate Professor, Division of Stem Cell Therapy, The Universiyt of Tokyo, Institute of Medical Science
Research focus
  • Stem cell
  • 1 Author merit

Education

PhD, University of Tsukuba, Japan, 2001

Publications

· Yamaguchi T. Hurdles to generating human islets in animals via blastocyst complementation. Curr Diab Rep. Accepted. (2019)

· Park MH, Choi BJ, Jeong MS, Lee JY, Jung IK, Park KH, Lee HW, Yamaguchi T, Marti HH, Lee BH, Schuchman EH, Jin HK, Bae JS. Characterization of the Subventricular-Thalamo-Cortical Circuit in the NP-C Mouse Brain, and New Insights Regarding Treatment. Mol Ther. May 16. pii: S1525-0016(19)30222-9. doi: 10.1016 (2019)

· Goto T, Hara H, Sanbo M, Masaki H, Sato H, Yamaguchi T, Hochi S, Kobayashi T, Nakauchi H, Hirabayashi M. Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats. Nat Commun. Feb 5;10(1):451. (2019).

· Mizuno N, Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H. Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing with Adeno-Associated Viral Vector. iScience. Nov 30;9:286-297 (2018).

· Park MH, Lee JY, Park KH, Jung IK, Kim KT, Lee YS, Ryu HH, Jeong Y, Kang M, Schwaninger M, Gulbins E, Reichel M, Kornhuber J, Yamaguchi T, Kim HJ, Kim SH, Schuchman EH, Jin HK, Bae JS. Vascular and Neurogenic Rejuvenation in Aging Mice by Modulation of ASM. Neuron. Oct 10;100(1):167-182.e9 (2018).

· Yamaguchi T, Sato H, Kobayashi T, Kato-Itoh M, Goto T, Hara H, Mizuno N, Yanagida A, Umino A, Hamanaka S, Suchy F, Masaki H, Ota Y, Hirabayashi M, Nakauchi H. An interspecies barrier to tetraploid complementation and chimera formation. Sci Rep. Oct 16;8(1):15289 (2018).

· Hamanaka S, Umino A, Sato H, Hayama T, Yanagida A, MizunoA, KobayashiT, Kasai M, Suchy F, Yamazaki S, Masaki H, Yamaguchi T, Nakauchi H. Generation of vascular endothelial cells and hematopoietic cells by blastocyst complementation. Stem Cell Reports. Oct 9;11(4):988-997 (2018).

· Oldani G, Peloso A, Vijgen S, Wilson EM, Slits F, Gex Q, Morel P, Delaune V, Orci LA, Yamaguchi T, Kobayashi T, Rubbia-Brandt L, Nakauchi H, Lacotte S, Toso C. Chimeric liver transplantation reveals interspecific graft remodelling. J Hepatol. Jul 19. pii: S0168-8278(18)32260-8 (2018).

· Yoshida A, Lee JK, Tomoyama S, Miwa K, Shirakawa K, Hamanaka S, Yamaguchi T, Nakauchi H, Miyagawa S, Sawa Y, Komuro I, Sakata Y. In vitro platform of allogeneic stem cell-derived cardiomyocyte transplantation for cardiac conduction defects. Europace. Sep 1;20(9):1553-1560 (2018).

· Suchy F, Yamaguchi T, Nakauchi H. iPSC-Derived Organs In Vivo: Challenges and Promise. Cell Stem Cell. Jan 4;22(1):21-24 (2018).

· Ren Y, Sekine-Kondo E, Shibata R, Kato-Itoh M, Umino A, Yanagida A, Satoh M, Inoue K, Yamaguchi T, Mochida K, Nakae S, Van Kaer L, Iwabuchi K, Nakauchi H, Watarai H. A Novel Mouse Model of iNKT Cell-deficiency Generated by CRISPR/Cas9 Reveals a Pathogenic Role of iNKT Cells in Metabolic Disease. Sci Rep. Oct 6;7(1):12765 (2017).

· Yamaguchi T, Sato H, Kato-Itoh M, Goto T, Hara H, Sanbo M, Mizuno N, Kobayashi T, Yanagida A, Umino A, Ota Y, Hamanaka S, Masaki H, Rashid ST, Hirabayashi M, Nakauchi H. Interspecies organogenesis generates autologous functional islets. Nature. Feb 9;542(7640):191-196 (2017).

· Masaki H, Kato-Itoh M, Takahashi Y, Umino A, Sato H, Ito K, Yanagida A, Nishimura T, Yamaguchi T, Hirabayashi M, Era T, Loh KM, Wu SM, Weissman IL, Nakauchi H. Inhibition of Apoptosis Overcomes Stage-Related Compatibility Barriers to Chimera Formation in Mouse Embryos. Cell Stem Cell. Nov 3;19(5):587-592 (2016).

· Hayama T, Yamaguchi T, Kato-Itoh M, Ishii Y, Mizuno N, Umino A, Sato H, Sanbo M, Hamanaka S, Masaki H, Hirabayashi M, Nakauchi H. Practical selection methods for rat and mouse round spermatids without DNA staining by flow cytometric cell sorting. Mol Reprod Dev. Jun;83(6):488-96 (2016)

· Otani S, Kakinuma S, Kamiya A, Goto F, Kaneko S, Miyoshi M, Tsunoda T, Asano Y, Kawai-Kitahata F, Nitta S, Nakata T, Okamoto R, Itsui Y, Nakagawa M, Azuma S, Asahina Y, Yamaguchi T, Koshikawa N, Seiki M, Nakauchi H, Watanabe M. Matrix metalloproteinase-14 mediates formation of bile ducts and hepatic maturation of fetal hepatic progenitor cells. Biochem Biophys Res Commun. Jan 22;469(4):1062-8 (2016).

· Lin HT, Masaki H, Yamaguchi T, Wada T, Yachie A, Nishimura K, Ohtaka M, Nakanishi M, Nakauchi H, Otsu M. An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils. Mol Ther Methods Clin Dev. Dec 9;2:15046 (2015).

· Yanagida A, Mizuno N, Yamazaki Y, Kato-Itoh M, Umino A, Sato H, Ito K, Yamaguchi T, Nakauchi H, Kamiya A. Investigation of bipotent differentiation of hepatoblasts using inducible diphtheria toxin receptor-transgenic mice. Hepatol Res. Jul;46(8):816-28 (2016).

· Ando M, Nishimura T, Yamazaki S, Yamaguchi T, Kawana-Tachikawa A, Hayama T, Nakauchi Y, Ando J, Ota Y, Takahashi S, Nishimura K, Ohtaka M, Nakanishi M, Miles JJ, Burrows SR, Brenner MK, Nakauchi H. A Safeguard System for Induced Pluripotent Stem Cell-Derived Rejuvenated T Cell Therapy. Stem Cell Reports. Oct 13;5(4):597-608 (2015).

· Yanagida A, Chikada H, Ito K, Umino A, Kato-Itoh M, Yamazaki Y, Sato H, Kobayashi T, Yamaguchi T, Nakayama KI, Nakauchi H, Kamiya A. Liver maturation deficiency in p57Kip2-/- mice occurs in a hepatocytic p57Kip2 expression-independent manner. Dev Biol. Jul 9. pii: S0012-1606(15)30037-3 (2015).

· Masaki H, Kato-Itoh M, Umino A, Sato H, Hamanaka S, Kobayashi T, Yamaguchi T, Nishimura K, Ohtaka M, Nakanishi M, Nakauchi H. Interspecific in vitro assay for the chimera-forming ability of human pluripotent stem cells. Development. Sep 15;142(18):3222-30 (2015).

· Murayama H, Masaki H, Sato H, Hayama T, Yamaguchi T, Nakauchi H. Successful Reprogramming of Epiblast Stem Cells by Blocking Nuclear Localization of β-Catenin. Stem Cell Reports. Jan 13;4(1):103-13 (2015).

· Hayama T, Yamaguchi T, Kato-ItohM, Hamanaka S, Kawarai M, Sanbo M, Tamura C, Lee YS, YanagidaA, Murayama H, Mizuno N, Umino A, Sato H, Yamazaki S, Masaki H, Kobayashi T, Hirabayashi M, Nakauchi H. Generation of mouse functional oocytes in rat by xeno-ectopic transplantation of primordial germ cells. Biol Reprod. Oct;91(4):89 (2014).

· Hirose S, Takayama N, Nakamura S, Nagasawa K, Ochi K, Hirata S, Yamazaki S, Yamaguchi T, Otsu M, Sano S, Takahashi N, Sawaguchi A, Ito M, Kato T, Nakauchi H, Eto K. Immortalization of erythroblasts by c-MYC and BCL-XL enables large-scale erythrocyte production from human pluripotent stem cells. Stem Cell Reports. 1, 499-508 (2013).

· Kon A, Shih LY, Minamino M, Sanada M, Shiraishi Y, Nagata Y, Yoshida K, Okuno Y, Bando M, Nakato R, Ishikawa S, Sato-Otsubo A, Nagae G, Nishimoto A, Haferlach C, Nowak D, Sato Y, Alpermann T, Nagasaki M, Shimamura T, Tanaka H, Chiba K, Yamamoto R, Yamaguchi T, Otsu M, Obara N, Sakata-Yanagimoto M, Nakamaki T, Ishiyama K, Nolte F, Hofmann WK, Miyawaki S, Chiba S, Mori H, Nakauchi H, Koeffler HP, Aburatani H, Haferlach T, Shirahige K, Miyano S, Ogawa S. Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms. Nat Genet. 45, 1232-1237 (2013) .

· Hamanaka S, Ooehara J, Morita Y, Ema H, Takahashi S, Miyawaki A, Otsu M, Yamaguchi T, Onodera M, Nakauchi H. Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method. Biochem Biophys Res Commun. 435, 586-591 (2013).

· Suzuki N, Yamazaki S, Yamaguchi T, Okabe M, Masaki H, Takaki S, Otsu M, Nakauchi H. Generation of Engraftable Hematopoietic Stem Cells From Induced Pluripotent Stem Cells by Way of Teratoma Formation. Mol Ther. 21, 1424-1431 (2013).

· Matsunari H, Nagashima H, Watanabe M, Umeyama K, Nakano K, Nagaya M, Kobayashi T, Yamaguchi T, Sumazaki R, Herzenberg LA, Nakauchi H. Blastocyst complementation generates exogenic pancreas in vivo in apancreatic cloned pigs. Proc Natl Acad Sci U S A. 110, 4557-4562 (2013).

· Yamaguchi T, Morikawa A & Miyoshi H. Comparison of gene-trapping efficiency between retroviral and lentiviral vectors in mouse embryonic stem cells. Biochem Biophys Res Commun. 425, 297-303 (2012).

· Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H. Development of an All-in-One Inducible Lentiviral Vector for Gene Specific Analysis of Reprogramming. PLoS One. 7, e41007 (2012).

· Kobayashi T, Kato-Itoh M, Yamaguchi T, Tamura C, Sanbo M, Hirabayashi M, & Nakauchi H. Identification of Rat Rosa26 Locus Enables Generation of Knock-in Rat Lines Ubiquitously Expressing tdTomato. Stem Cells Dev. 21, 2981-2986. (2012).

· Usui J, Kobayashi T, Yamaguchi T, Knisely AS, Nishinakamura R, & Nakauchi H. Generation of kidney from pluripotent stem cells via blastocyst complementation. Am J Pathol. 180, 2417-2426 (2012).

· Suzuki N, Yamazaki S, Ema H, Yamaguchi T, Nakauchi H, & Takaki S. Homeostasis of hematopoietic stem cells regulated by the myeloproliferative disease associated-gene product Lnk/Sh2b3 via Bcl-xL. Exp Hematol. 40, 166-174, (2012).

· Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, Taketo M.M, Karlsson S, Iwama A, & Nakauchi H. Nonmyelinating schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche. Cell, 147, 1146-1158 (2011).

· Yoshida K, Sanada M, Shiraishi Y, Nowak D, Nagata Y, Yamamoto R, Sato Y, Sato-Otsubo A, Kon A, Nagasaki M, Chalkidis G, Suzuki Y, Shiosaka M, Kawahata R, Yamaguchi T, Otsu M, Obara N, Sakata-Yanagimoto M, Ishiyama K, Mori H, Nolte F, Hofmann WK, Miyawaki S, Sugano S, Haferlach C, Koeffler HP, Shih LY, Haferlach T, Chiba S, Nakauchi H, Miyano S, & Ogawa S. Frequent pathway mutations of splicing machinery in myelodysplasia. Nature. 478, 64-69 (2011).

· Hamanaka S, Yamaguchi T, Kobayashi T, Kato-Ito, M, Yamazaki S, Sato H, Umino A, Wakiyama Y, Arai M, Sanbo M, Hirabayashi M, & Nakauchi H. Generation of germline-competent rat induced pluripotent stem cells. PLoS One. 6, e22008 (2011).

· Takayama N, Nishimura S, Nakamura S, Shimizu T, Ohnishi R, Endo H, Yamaguchi T, Otsu M, Nishimura K, Nakanishi M, Sawaguchi A, Nagai R, Takahashi K, Yamanaka S, Nakauchi H, & Eto K. Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells. J Exp Med. 207, 2817-2830 (2010).

· Kobayashi T, Yamaguchi T, Hamanaka S, Kato-Itoh M, Yamazaki Y, Ibata M, Sato H, Lee YS, Usui J, Knisely AS, Hirabayashi M, & Nakauchi H. Generation of rat pancreas in mouse by interspecific blastocyst injection of pluripotent stem cells. Cell. 142, 787-799 (2010).

· Watanabe N, Nishihara Y, Yamaguchi T, Koito A, Miyoshi H, Kakeya H, & Osada H. Fumagillin suppresses HIV-1 infection of macrophages through the inhibition of Vpr activity. FEBS Lett. 580, 2598-2602 (2006).

· Score PR, Belur LR, Frandsen JL, Geurts JL, Yamaguchi T, Somia NV, Hackett PB, Largaespada DA, & McIvor RS. Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression. Mol Ther. 13, 617-624 (2006).

· Agarwal S, Nikolai B, Yamaguchi T, Lech P, & Somia NV. Construction and use of retroviral vectors encoding the toxic gene barnase. Mol Ther. 14, 555-563 (2006).

· Agarwal S, Harada J, Schreifels J, Lech P, Nikolai B, Yamaguchi T, Chanda SK, & Somia NV. Isolation, characterization, and genetic complementation of a cellular mutant resistant to retroviral infection. Proc Natl Acad Sci U S A. 103, 15933-15938 (2006).
1 Protocol published
CRISPR/Cas9 + AAV-mediated Intra-embryonic Gene Knocking in Mice
Intra-embryo genome editing by CRISPR/Cas9 has enabled rapid generation of gene knockout animals. However, large fragment knock-in directly into embryos’ genome is still difficult, especially without microinjection of donor DNA. Viral vectors are ...
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