Michelle Facette —BIO-PROTOCOL

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Janette Mendoza University of New Mexico, United States
1 protocol

Qiong Nan Biology department, University of Massachusetts, USA
1 protocol

Michelle Facette

Faculty, Biology, University of Massachusetts, Amherst, United States

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1 Author merit
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1 Protocol published

Double Labeling of Microtubules and Actin Filaments in Maize Leaf Division Zone

Authors: Qiong Nan, Janette Mendoza and Michelle Renee Facette, date: 06/20/2019, view: 2399, Q&A: 1

Maize leaves grow in a gradient. The base of young maize leaves contains a high proportion of symmetrically and asymmetrically dividing cells, making the leaf an effective model system for the study of cell division. Here, we describe a double ...

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克东翚

广西大学

When preparing the Fix solution, it is stated that AlexaFluor 568-Phalloidin needs to be added, and Materials and Reagents only need to have Goat anti-mouse AlexaFluor 568 and AlexaFluor 488-Phalloidin, so here is the mistake of writing AlexaFluor 488-Phalloidin as AlexaFluor 568-Phalloidin ?

10/30/2020 5:27:48 PM Reply

Michelle Facette

Biology, University of Massachusetts, Amherst, United States

Yes - thank you for catching this and our apologies. The fluor on the Phalloidin should be different than the fluor on the antibody. Thus, it should state AlexaFluor 488-Phalloidin is added (if using Goat anti-mouse AlexaFluor 568). These two colors can be reversed if you wish - we find the brighter actin staining easier to see - but either way it will work, as long as the microtubules and actin filaments are labelled with different colors.

2020-11-04 06:01:55

克东 翚

广西大学

Michelle Facette

Biology, University of Massachusetts, Amherst, United States

克东翚