Decai Mao 1 protocol

Ruibao Zhu 1 protocol

Jin Sun 3 protocols

Rong-Gang Xu 3 protocols

Jian-Quan Ni
  • 清华大学医学院, 北京
  • 4 Author merit

Education

Ph.D, Basel University, Switzerland, 2006

Current position

Professor, School of Medicine, Tsinghua University, China

Publications

  1. Y Gao; Y Mao; R.G Xu; R Zhu; M Zhang; J Sun; D Shen; P Peng; T Xie#; J.Q Ni# Defining Gene Networks Controlling the Maintenance and Function of the Differentiation Niche by an in vivo Systematic RNAi Screen, J Genet Genomics. In press 
  2. Qiao HH, Wang F, Xu RG, Sun J, Zhu R, Mao D, Ren X, Wang X, Jia Y, Peng P, Shen D, Liu LP, Chang Z, Wang G, Li S, Ji JY, Liu Q, Ni JQ# An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila, Nature communications, 2018 Oct 8;9(1):4160. 
  3. Zheng Y, Xue Y, Ren X, Liu M, Li X, Jia Y, Niu Y, Ni J.Q#, Zhang Y#, Ji JY#. The Lysine Demethylase dKDM2 Is Non-essential for Viability, but Regulates Circadian Rhythms in Drosophila. Front Genet. 2018 Sep 4;9:354. 
  4. Sapar ML, Ji H, Wang B, Poe AR, Dubey K, Ren X, Ni J.Q, Han C#. Phosphatidylserine Externalization Results from and Causes Neurite Degeneration in Drosophila. Cell Rep. 2018 Aug 28;24(9):2273-2286. 
  5. 徐荣刚,王霞,王芳,孙锦,毛德才,乔欢欢,贾豫,朱芮葆,彭娉,刘鲁萍,倪建泉# 果蝇研究技术与资源的开发,ACTA LABORATORIUM ANIMAL SCIENTIA SINICA, August 2018, Vol. 26, No. 4 
  6. Ke H, Feng Z, Liu M, Sun T, Dai J, Ma M, Liu LP, Ni JQ, Pastor-Pareja JC#. Collagen secretion screening in Drosophila supports a common secretory machinery and multiple Rab requirements. J Genet Genomics. 2018 Jun 1. 
  7. 王霞,孙锦,王芳,乔欢欢,毛德才,倪建泉# 模式动物果蝇新品系开发与应用, 医学科技,DOI:10.3772/j.issn.1673-6516.2018.07.022 
  8. Fan X, Zhao J, Ren F, Wang Y, Feng Y, Ding L, Zhao L, Shang Y, Li J, Ni JQ, Jia B, Liu Y, Chang Z#. Dimerization of p15RS mediated by a leucine zipper-like motif is critical for its inhibitory role on Wnt signaling. J Biol Chem. 2018 May 18;293(20):7618-7628. 
  9. 贾豫,徐荣刚,孙锦,倪建泉# 高效特异的CRISPR/dCas9转录激活系统——flySAM系统, Hereditas, 2018 年5 月, 40(5): 425―427 
  10. Y Jia, R.G Xu, X Ren, B Ewen-Campen, R Rajakumar, J Zirin, D Yang-Zhou, R Zhu, F Wang, DMao, P Peng, H.H Qiao, X Wang, L.P Liu, B Xu, J.Y Ji, Q Liu, J Sun#, N Perrimon#, Ni J.Q#, Next-generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM, Proc Natl Acad Sci USA. 2018 May 1;115(18):4719-4724. 
  11. Ewen-Campen, B., Yang-Zhou, D., Fernandes, V.R., Gonzalez, D.P., Liu, L.P., Tao, R., Ren, X., Sun, J., Hu, Y., Zirin, J., Mohr, S.E., Ni J.Q#, and Perrimon, N#. Optimized strategy for in vivo Cas9-activation in Drosophila. Proc Natl Acad Sci USA. 2017 Aug 29;114(35):9409-9414. 
  12. Ren X, Holsteens K, Li H, Sun J, Zhang Y, Liu LP, Liu Q, Ni J.Q#. Genome editing in Drosophila melanogaster: from basic genome engineering to the multipurpose CRISPR-Cas9 system. Sci China Life Sci. 2017 May;60(5):476-489. 
  13. Yang, Z., Sun, J., Hu, Y., Wang, F., Wang, X., Qiao, H.H., Xu, J., Mao, D., Ren, X., Pan, L.X., Xu, R.G., Xu, B.W., Zhang, Y., Li, H., Miao, W., Hu, Y., Chang, Z., Wang, D., Li, H., Chang, Z., Liu, L.P., Liu, Q., Ni, J.Q#. Histone H1 defect in escort cells triggers germline tumor in Drosophila ovary. Developmental biology. 2017 Apr 1;424(1):40-49. 
  14. Ma X, Han Y, Song X, Do T, Yang Z, Ni JQ, Xie T#. DNA damage-induced Lok/CHK2 activation compromises germline stem cell self-renewal and lineage differentiation. Development. 
  15. Xiong XP, Kurthkoti K, Chang KY, Li JL, Ren X, Ni JQ, Rana TM, Zhou R#. miR-34 Modulates Innate Immunity and Ecdysone Signaling in Drosophila. PLoS Pathog, 2016,12(11):e1006034 
  16. 王霞, 乔欢欢, 潘丽霞, 任兴杰, 孙锦, 徐荣刚, 刘鲁萍, 贺争鸣, 倪建泉# 果蝇新品系开发与种质资源保存,LABORATORY ANIMAL SCIENCE,Vol.33 No.3 June 2016 
  17. Lu T, Wang S, Gao Y, Mao Y, Yang Z, Liu L, Song X, Ni JQ#, Xie T#. COP9-Hedgehog Axis Regulates the Function of the Germline Stem Cell Progeny Differentiation Niche in the Drosophila Ovary, Development. 2015 Dec 15;142(24):4242-52. 
  18. Sun J, Wei HM, Xu J, Chang JF, Yang Z, Ren X, Lv WW, Liu LP, Pan LX, Wang X, Qiao HH, Zhu B, Ji JY, Yan D, Xie T, Sun FL#, Ni JQ#. Histone H1-Mediated Epigenetic Regulation Controls Germline Stem Cell Self-Renewal by Modulating H4K16 Acetylation, Nature Communications. 2015 Nov 19;6:8856. 
  19. Fu Z, Geng C, Wang H, Yang Z, Weng C, Li H, Deng L, Liu L, Liu N, Ni JQ, Xie T# Twin Promotes the Maintenance and Differentiation of Germline Stem Cell Lineage through Modulating Multiple Pathways, Cell Reports. 2015, Nov 5. 
  20. Pan L, Xie W, Li KL, Yang Z, Xu J, Zhang W, Liu LP, Ren X, He Z, Wu J, Sun J, Wei HM, Wang D, Xie W, Li W, Ni JQ#, Sun FL#. Heterochromatin remodeling by CDK12 contributes to learning in Drosophila, Proc Natl Acad Sci USA. 2015 Nov 10;112(45):13988-93.
  21. Wang S, Gao Y, Song X, Ma X, Zhu X, Mao Y, Yang Z, Ni JQ, Li H, Malanowski KE, Anoja P, Park J, Haug J, Xie T#. Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche, ELife. October 9, 2015
  22. Perkins LA, Holderbaum L, Tao R, Hu Y, Sopko R, McCall K, Yang-Zhou D, Flockhart I, Binari R, Shim HS, Miller A, Housden A, Foos M, Randkelv S, Kelley C, Namgyal P, Villalta C, Liu LP, Jiang X, Huan-Huan Q, Wang X, Fujiyama A, Toyoda A, Ayers K, Blum A, Czech B, Neumuller R, Yan D, Cavallaro A, Hibbard K, Hall D, Cooley L, Hannon GJ, Lehmann R, Parks A, Mohr SE, Ueda R, Kondo S, Ni JQ, Perrimon N#. The Transgenic RNAi Project at Harvard Medical School: Resources and Validation,Genetics. 2015 Aug 28. pii: Genetics.115.180208. 
  23. Xie XJ, Hsu FN, Gao X, Xu W, Ni JQ, Xing Y, Huang L, Hsiao HC, Zheng H, Wang C, Zheng Y, Xiaoli AM, Yang F, Bondos SE, Ji JY#. CDK8-Cyclin C Mediates Nutritional Regulation of Developmental Transitions through the Ecdysone Receptor in Drosophila, PLoS Biology. 2015 Jul 29;13(7):e1002207. 
  24. Zang Y, Wan M, Liu M, Ke H, Ma S, Liu LP, Ni JQ, Pastor-Pareja JC#. Plasma membrane overgrowth causes fibrotic collagen accumulation and immune activation in Drosophila adipocytes, Elife. 2015 Jun 19;4:e07187. 
  25. Xu J, Ren X, Sun J, Wang X, Qiao HH, Xu BW, Liu LP, Ni JQ#, A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila, J Genet Genomics. 2015 Apr 20;42(4):141-9. 
  26. Ren X, Yang Z, Xu J, Sun J, Mao D, Hu Y, Yang SJ, Qiao HH, Wang X, Hu Q, Deng P, Liu LP, Ji JY, Li JB#, Ni JQ#, Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila. Cell Reports. 2014 Nov 6;9(3):1151-62. 
  27. Ren X, Yang Z, Mao D, Chang Z, Qiao HH, Wang X, Sun J, Hu Q, Cui Y, Liu LP, Ji JY, Xu J, Ni JQ#. Performance of the Cas9 Nickase System in Drosophila melanogaster. G3 (Bethesda). 2014 Aug 15;4(10):1955-62. 
  28. Pan L, Wang S, Lu T, Weng C, Song X, Park JK, Sun J, Yang ZH, Yu J, Tang H, McKearin DM, Chamovitz DA, Ni JQ, Xie T#. Protein competition switches the function of COP9 from self-renewal to differentiation. Nature. 2014 Oct 9;514(7521):233-6. 
  29. Zheng Y, Hsu FN, Xu W, Xie XJ, Ren X, Gao X, Ni JQ, Ji JY#. A Genetic Analysis of the Lysine Demethylase KDM2 in Drosophila melanogaster. Mech Dev. 2014 Aug;133:36-53. 
  30. Ma X, Wang S, Do T, Song X, Inaba M, Nishimoto Y, Liu LP, Gao Y, Mao Y, Li H, McDowell W, Park J, Malanowski K, Peak A, Perera A, Li H, Gaudenz K, Haug J, Yamashita Y, Lin H, Ni JQ, Xie T#. Piwi is required in multiple cell types to control germline stem cell lineage development in the Drosophila ovary. PLoS One. 2014 Mar 21;9(3):e90267. 
  31. Ren X, Sun J, Housden BE, Hu Y, Roesel C, Lin S, Liu LP, Yang Z, Mao D, Sun L, Wu Q, Ji JY, Xi J, Mohr SE, Xu J#, Perrimon N#, Ni JQ#. An optimized gene editing technology for Drosophila melanogaster using germline-specific Cas9. Proc Natl Acad Sci USA. 2013 Nov 19;110(47):19012-7. 
  32. Y Yang, L Hou, Y Li, Jianquan Ni and L Liu#, Neuronal necrosis and spreading death in a Drosophila genetic model, Cell Death and Disease, 2013, (2013) 4, e723. 
  33. Lv WW, Wei HM, Wang DL, Ni JQ, Sun FL#. Depletion of histone deacetylase 3 antagonizes PI3K-mediated overgrowth through the acetylation of histone H4 at lysine 16. J Cell Sci. 2012 Nov 15;125(Pt 22):5369-78. 
  34. Bejarano F, Bortolamiol-Becet D, Dai Q, Sun K, Saj A, Chou YT, Raleigh DR, Kim K, Ni JQ, Duan H, Yang JS, Fulga TA, Van Vactor D, Perrimon N, Lai EC#. A genomewide transgenic resource for conditional expression of Drosophila microRNAs. Development. 2012 Aug;139(15):2821-31. 
  35. Zhao X, Feng D, Wang Q, Abdulla A, Xie XJ, Zhou J, Sun Y, Yang ES, Liu LP, Vaitheesvaran B, Bridges L, Kurland IJ, Strich R, Ni JQ, Wang C, Ericsson J, Pessin JE, Ji JY#, Yang F#. A Conserved Function of CDK8 in Suppressing SREBP-dependent Lipogenesis. Journal of Clinical Investigation. 2012 Jul 2;122(7):2417-27. 
  36. Ni JQ*, Zhou R, Czech B, Liu LP, Holderbaum L, Yang-Zhou D, Shim HS, Tao R, Handler D, Karpowicz P, Binari R, Booker M, Brennecke J, Perkins LA, Hannon GJ, Perrimon N#. A genome-scale shRNA resource for transgenic RNAi in Drosophila . Nature Methods. 2011 May;8(5):405-7. 
  37. Perrimon N#, Ni JQ, Perkins L. In vivo RNAi: Today and Tomorrow. Cold Spring Harbor Perspectives in Biology. 2010 Aug 1;2(8):a003640. 
  38. Ni JQ, Liu LP, Binari R, Hardy R, Shim HS, Cavallaro A, Booker M, Pfeiffer BD, Markstein M, Wang H, Villalta C, Laverty TR, Perkins LA, Perrimon N#. A Drosophila Resource of transgenic RNAi lines for Neurogenetics. Genetics. 2009 Aug; 182(4):1089-100. 
  39. Jianwu Bai, Richard Binari, Jian-Quan Ni, Marina Vijayakanthan, Hong-Sheng Li and Norbert Perrimon#. RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Development. 2008 Apr;135(8):1439-49. 
  40. Jian-Quan Ni, Michele Markstein, Richard Binari, Barret Pfeiffer, Lu-Ping Liu, Christians Villalta, Matthew Booker, Lizabeth Perkins, and Norbert Perrimon#. Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Nature Methods. 2008 January; 5(1): 49–51. 
  41. Jian-Quan Ni, Lu-Ping Liu, Daniel Hess, and Fang-Lin Sun#. Drosophila ribosomal proteins are associated with chromatin through histone H1 and suppress global transcription. Genes Development. 2006 Jul 15;20(14):1959-73.
  42. Cen X, Ni Jianquan, Tan T, Liu X, Li C, Chen J, Huang Y, Zhu S, Bi Q#. Investigation on recombinant hirudin via oral route. Peptides. 2006 Apr;27(4):836-40. Epub 2006 Feb 13. 
  43. Lu-Ping Liu#, Jian-Quan Ni#, Yan-Dong Shi, Edward. J. Oakeley & Fang-Lin Sun#. Sex-specific role of Drosophila melanogaster HP1 in regulating chromatin structure and gene transcription. Nature Genetics. 2005 Dec;37(12):1361-6. 
  44. Filomena De Lucia, Jian-Quan Ni, Catherine Vaillant, and Fang-Lin Sun#. HP1 modulates the transcription of cell-cycle regulators in Drosophila melanogaster. Nucleic Acids Res. 2005. 33(9): 2852-8.
4 Protocols published
pNP Transgenic RNAi System Manual in Drosophila
Authors:  Fang Wang, Huan-Huan Qiao, Rong-Gang Xu, Jin Sun, Ruibao Zhu, Decai Mao and Jian-Quan Ni , date: 02/05/2019, view: 186, Q&A: 0
Much of our knowledge about the mechanisms underlying biological processes relies on genetic approaches, whereby gene activity is reduced and the phenotypic consequences of perturbation are analyzed in detail. For functional genomic studies, a ...
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