Kurt Krause Biochemistry, Otago University, New Zealand, New Zealand,
1 protocol

Roman Mortuza 1 protocol

Helen Kim Opel-Reading
  • Biochemistry, Otago University, New Zealand, New Zealand,
Research focus
  • Microbiology
  • 1 Author merit


BSc(Hons), University of Otago/Biochemistry, New Zealand, 2011

Current position

Assistant Research Fellow, Biochemistry, University of Otago, New Zealand


  1. Mortuza, R., Aung, H.L., Taiaroa, G., Opel-Reading, H.K., Kleffmann, T., Cook, G.M., Krause, K.L. Overexpression of a newly identified d-amino acid transaminase in Mycobacterium smegmatis complements glutamate racemase deletion. (2018) Molecular Microbiology, 107 (2), pp. 198-213. Cited 3 times. DOI: 10.1111/mmi.13877
  2. Nakatani, Y., Opel-Reading, H.K., Merker, M., Machado, D., Andres, S., Kumar, S.S., Moradigaravand, D., Coll, F., Perdigão, J., Portugal, I., Schön, T., Nair, D., Devi, K.R.U., Kohl, T.A., Beckert, P., Clark, T.G., Maphalala, G., Khumalo, D., Diel, R., Klaos, K., Aung, H.L., Cook, G.M., Parkhill, J., Peacock, S.J., Swaminathan, S., Viveiros, M., Niemann, S., Krause, K.L., Köser, C.U. Role of alanine racemase mutations in Mycobacterium tuberculosis D-cycloserine resistance. (2017) Antimicrobial Agents and Chemotherapy, 61 (12), art. no. e01575, . Cited 2 times. DOI: 10.1128/AAC.01575-17
  3. Poen, S., Nakatani, Y., Opel-Reading, H.K., Lasse, M., Dobson, R.C.J., Krause, K.L. Exploring the structure of glutamate racemase from Mycobacterium tuberculosis as a template for anti-mycobacterial drug discovery. (2016) Biochemical Journal, 473 (9), pp. 1267-1280. Cited 7 times. DOI: 10.1042/BCJ20160186
  4. Wilkinson, M.E., Nakatani, Y., Staals, R.H.J., Kieper, S.N., Opel-Reading, H.K., McKenzie, R.E., Fineran, P.C., Krause, K.L. Structural plasticity and in vivo activity of Cas1 from the type I-F CRISPR-Cas system. (2016) Biochemical Journal, 473 (8), pp. 1063-1072. Cited 4 times. DOI: 10.1042/BCJ20160078
  5. Davis, E., Scaletti-Hutchinson, E., Opel-Reading, H., Nakatani, Y., Krause, K.L. The structure of alanine racemase from Acinetobacter baumannii. (2014) Acta Crystallographica Section:F Structural Biology Communications, 70, pp. 1199-1205. Cited 5 times. DOI: 10.1107/S2053230X14017725
1 Protocol published
Detection of D-glutamate Production from the Dual Function Enzyme, 4-amino-4-deoxychorismate Lyase/D-amino Acid Transaminase, in Mycobacterium smegmatis
Authors:  Helen K. Opel-Reading, Roman Mortuza and Kurt L. Krause, date: 01/05/2019, view: 3669, Q&A: 0
D-amino acid transaminase (D-AAT) is able to synthesize both D-glutamate and D-alanine, according to the following reaction: D-alanine + α-ketoglutarate ⇌ D-glutamate + pyruvate. These two D-amino acids are essential components of the peptidoglycan ...
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