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A peer-reviewed protocol journal. No publication fee; no access fee.
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Jillian Carr
College of Medicine and Public Health, Flinders University, Australia
1 protocol
David Gordon
College of Medicine and Public Health, Flinders University, South Australia, South Australia,
1 protocol
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Sheila Cabezas Falcon
College of Medicine and Public Health, Flinders University, South Australia, South Australia,
Research focus
Immunology
Contributions
1
Author merit
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1 Protocol published
Quantitation of Regulatory Activity for the Complement Alternative Pathway Using an Adaptation of the AP
50
in vitro
Assay
Authors:
Sheila Cabezas Falcon
,
David L Gordon
and
Jillian M Carr
,
date:
12/20/2018,
view:
2927,
Q&A:
2
Complement pathways function to identify and remove pathogens and infected cells. There are three complement pathways: the classical, lectin and alternative pathway (AP). While all pathways are activated following pathogen stimuli, the AP is ...
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Zhang Liming
中南大学
The AP buffer needs to be added NaCl and Glucose to ensure the osmotic pressure.
10/17/2019 10:06:55 PM
Reply
Jillian Carr
College of Medicine and Public Health, Flinders University, Australia
correction - NaCl but no glucose. Tablets are from oxoid for complement fixation test http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=BR0016&cat=&sec=1&c=UK&lang=EN
2019-10-23 15:46:14
Jillian Carr
College of Medicine and Public Health, Flinders University, Australia
Hi Zhang
You are correct, but in our protocol the veronal buffer is made up from a barbitone tablet that already contains glucose and NaCl
Jill
2019-10-23 15:38:26
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