Sheila Cabezas Falcon —BIO-PROTOCOL

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Jillian Carr College of Medicine and Public Health, Flinders University, Australia
1 protocol

David Gordon College of Medicine and Public Health, Flinders University, South Australia, South Australia,
1 protocol

Sheila Cabezas Falcon

College of Medicine and Public Health, Flinders University, South Australia, South Australia,

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Quantitation of Regulatory Activity for the Complement Alternative Pathway Using an Adaptation of the AP₅₀ in vitro Assay

Authors: Sheila Cabezas Falcon, David L Gordon and Jillian M Carr, date: 12/20/2018, view: 1401, Q&A: 2

Complement pathways function to identify and remove pathogens and infected cells. There are three complement pathways: the classical, lectin and alternative pathway (AP). While all pathways are activated following pathogen stimuli, the AP is ...

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Zhang Liming

中南大学

The AP buffer needs to be added NaCl and Glucose to ensure the osmotic pressure.

10/17/2019 10:06:55 PM Reply

Jillian Carr

College of Medicine and Public Health, Flinders University, Australia

correction - NaCl but no glucose. Tablets are from oxoid for complement fixation test http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=BR0016&cat=&sec=1&c=UK&lang=EN

2019-10-23 15:46:14

Jillian Carr

College of Medicine and Public Health, Flinders University, Australia

Hi Zhang
You are correct, but in our protocol the veronal buffer is made up from a barbitone tablet that already contains glucose and NaCl
Jill

2019-10-23 15:38:26