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Nazish Sayed
1 protocol
Christopher Chen
Stanford Cardiovascular Institut e, Stanford University School of Medicine, USA, USA,
1 protocol
Chun Liu
Stanford University
1 protocol
View all coauthors >
Linlin Cheng
Research focus
Stem cell
Contributions
1
Author merit
Reviewer merit
Editor merit
1 Protocol published
Generation of Endothelial Cells from Human Induced Pluripotent Stem Cells
Authors:
Chun Liu
,
Linling Cheng
,
Christopher Li Chen
and
Nazish Sayed
,
date:
11/20/2018,
view:
3132,
Q&A:
4
Endothelial cells (ECs), as important constituent of blood vessel are crucial to maintaining vascular tone, and any dysfunction in ECs could lead to many diseases of the vascular system. Due to limited availability of human samples, most of our ...
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laura chomette
free university of brussels
I have another question. How much B27 do you add to the RPMI? Is it the same as for cardiomyocytes protocols? 10%?
Thank you very much for your help
3/8/2019 12:38:46 AM
Reply
laura chomette
free university of brussels
Thanks!
2019-03-14 08:13:08
Chun Liu
Stanford University
10 ml B27 minus insulin into 500 ml RPMI medium, 2%
2019-03-14 00:19:49
laura chomette
free university of brussels
or 2%?
2019-03-08 01:32:08
following
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