Tânia Simões University of Cologne Cologne
2 protocols

Ira Buntenbroich University of Cologne Cologne
1 protocol

Fabian den Brave Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Germany, Germany,
1 protocol

Ramona Schuster Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases, University of Cologne, Germany, Germany,
1 protocol

Mafalda Escobar-Henriques
  • Faculty, University of Cologne Cologne
  • 2 Author merit


PhD, Bordeaux2, France, 2001

Current position

Research group leader, Institute for Genetics, University of Cologne, Germany


  1. Shai N, Yifrach E, van Roermund CWT, Cohen N, Bibi C, IJlst L, Cavellini L, Meurisse J, Schuster R, Zada L, Mari MC, Reggiori FM, Hughes AL, Escobar-Henriques M, Cohen MM, Waterham HR, Wanders RJA,  Schuldiner M, Zalckvar E. 2018. Systematic mapping of contact sites reveals tethers and a function for the peroxisome-mitochondria contact. Nature communications 9:1761.

  2. Simões T, Schuster R, den Brave F, Escobar-Henriques M. 2018. Cdc48 regulates a deubiquitylase cascade critical for mitochondrial fusion, Elife. Jan 8 e30015. Selected for elife digestion

  3. Anton F, Dittmar G, Langer T, Escobar-Henriques M. 2013. Two deubiquitylases act on mitofusin and regulate mitochondrial fusion along independent pathways. Molecular Cell 49:487–498. Highlighted in Molecular Cell, in Nature Rev. Mol. Cell Biol. and in F1000.

  4. Anton F, Fres JM, Schauss A, Pinson B, Praefcke GJ, Langer T, Escobar-Henriques M. 2011. Ugo1 and Mdm30 act sequentially during Fzo1-mediated mitochondrial outer membrane fusion. Journal of Cell Science 124:1126–1135.

  5. Dürr M, Escobar-Henriques M, Merz S, Geimer S, Langer T and Westermann B. 2006. Non-redundant roles of mitochondria-associated F-box proteins, Mfb1 and Mdm30, in maintenance of mitochondrial morphology in yeast, Mol. Biol. Cell 17: 3745-3755.

  6. Escobar-Henriques M, Westermann B, Langer T. 2006. Regulation of mitochondrial fusion by the F-box protein Mdm30 involves proteasome-independent turnover of Fzo1. The Journal of Cell Biology 173:645–650.

  7. Escobar-Henriques M, Daignan-Fornier B, Collart MA. 2003. The critical cis-acting element required for IMD2 feedback regulation by GDP is a TATA box located 202 nucleotides upstream of the transcription start site. Mol. Cell. Biol. 1763(17), 6267-6278.

  8. Escobar-Henriques M, Collart MA, Daignan-Fornier B. 2003. Transcription initiation of the yeast IMD2 gene is abolished in response to nutrient limitation through a sequence in its coding region. Molecular and Cellular Biology. 23:6279-90.

  9. Escobar-Henriques M, Balguerie A, Monribot C, Boucherie H, Daignan-Fornier B. 2001. Proteome analysis and morphological studies reveal multiple effects of the immunosuppressive drug mycophenolic acid specifically resulting from guanylic nucleotide depletion. Journal of Biological Chemistry. 276:46237-46242.

  10. Escobar-Henriques M, Daignan-Fornier B. 2001. Transcriptional regulation of the yeast GMP synthesis pathway by its end products. Journal of Biological Chemistry. 276:1523-1530.


  11. Escobar-Henriques M. 2014. Mitofusins: ubiquitylation promotes fusion. Cell Research 4:387–388.

  12. Escobar-Henriques M, Langer T. 2014. Dynamic survey of mitochondria by ubiquitin. EMBO Reports 15:231–243.

  13. Escobar-Henriques M, Anton F. 2013. Mechanistic perspective of mitochondrial fusion: tubulation vs. fragmentation. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1833:162–175.

  14. Escobar-Henriques M, Langer T. 2006. Mitochondrial shaping cuts. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1763:422-429.

3 Protocols published
Analysis of Protein Stability by Synthesis Shutoff
Authors:  Ira Buntenbroich, Tânia Simões and Mafalda Escobar-Henriques, date: 11/20/2021, view: 530, Q&A: 0

In this protocol, we describe the analysis of protein stability over time, using synthesis shutoff. As an example, we express HA-tagged yeast mitofusin Fzo1 in Saccharomyces cerevisiae and inhibit translation via cycloheximide (CHX). Proteasomal

Separation and Visualization of Low Abundant Ubiquitylated Forms
Authors:  Ramona Schuster, Tânia Simões, Fabian den Brave and Mafalda Escobar-Henriques, date: 11/20/2018, view: 3513, Q&A: 0
In this protocol we describe the separation and visualization of ubiquitylated forms of the yeast mitofusin Fzo1 by Western blot. To this aim, we express HA-tagged Fzo1 in Saccharomyces cerevisiae, break the cells to extract a ...
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