Andrea Puhar The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
4 protocols

Atin Sharma
  • PostDoc Researcher, The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University
Research focus
  • Microbiology
  • Shigella virulence
  • 2 Author merit
Lab Protocol Hub

Education

PhD, Indian Institute of Technology Roorkee, India, 2018

Lab information

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar/research-andrea-puhar.html

Publications

2 Protocols published
Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
Authors:  Atin Sharma and Andrea Puhar, date: 07/05/2019, view: 425, Q&A: 0
Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to ...
Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri
Authors:  Atin Sharma and Andrea Puhar, date: 07/05/2019, view: 502, Q&A: 0
Shigella flexneri is an intracellular bacterial pathogen that gains access to the gut epithelium using a specialized Type III Secretion System (T3SS). Various determinants mediating this invasive infection have been experimentally verified ...
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