Edward L LaGory —BIO-PROTOCOL

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Yu Liu Department of Biology and Biochemistry, University of Houston, the United States of America
2 protocols

Amato Giaccia Radiation Oncology, Stanford University, USA, USA,
1 protocol

Kaushik Thakkar Radiation Oncology, Stanford University, USA
1 protocol

Neha Tandon Department of Biology and Biochemistry, University of Houston, USA, USA,
1 protocol

Edward L LaGory

Radiation Oncology, Stanford University, USA, USA,

Research focus

Molecular biology

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Generation of Stable Expression Mammalian Cell Lines Using Lentivirus

Authors: Neha Tandon, Kaushik N. Thakkar, Edward L LaGory, Yu Liu and Amato J Giaccia, date: 11/05/2018, view: 11913, Q&A: 1

Lentiviruses are used very widely to generate stable expression mammalian cell lines. They are used for both gene down-regulation (by using shRNA) or for gene up-regulation (by using ORF of gene of interest). The technique of generating stable cell ...

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立奇殷

18340869839

Without resistance gene screening, when to use fluorescence microscopy to see transfection efficiency

6/18/2021 1:03:05 AM Reply

Yu Liu

Department of Biology and Biochemistry, University of Houston, the United States of America

For plasmid transfection into 293T cells, 48 hours will give bright fluorescence. For lentiviral transduction into target cells, I usually wait for 72hrs before checking for efficiency.

2021-06-25 13:51:34

磊周

西南医院

Generally, 48 hours after lentivirus infection is enough to observe bright fluorescence under microscope or using flow cytometry.

2021-06-20 21:02:40

立奇 殷

18340869839

Yu Liu

Department of Biology and Biochemistry, University of Houston, the United States of America

磊 周

西南医院

立奇殷

磊周