Hi, I'd like to ask some questions about reverse transcription. In this protocol and other articles I have read, the same quality of total RNA was used at different time points for reverse transcription.If my target mRNA decays at the same rate as total RNA, am I not getting its half-life? Because it is in equal proportion to an equal amount of total RNA. Did I misunderstand?Thank you. 5/24/2020 11:23:11 AM Reply